Effects of YB-1 on rat mesangial cells proliferation and TGF-β1 expression
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摘要:
目的:观察Y-box结合蛋白-1(Y-box binding protein 1,YB-1)对体外培养的大鼠肾小球系膜细胞(MC)增生及分泌转化生长因子-β1(TGF-β1)的影响-方法:体外培养大鼠肾小球系膜细胞株(HBZY-1),构建YB-1真核表达载体,转染系膜细胞,应用四甲基偶氮唑蓝(MTT)法及细胞计数法检测大鼠MC过表达YB-1后的增生情况,双抗体夹心ELISA法检测细胞培养上清液中TGF-β1分泌,real-time PCR法测定细胞TGF-β1基因相对表达量-结果:YB-1过表达后MC增生和TGF-β1基因表达增加,同时MC分泌TGF-β1增加-结论:YB-1能促进MC增生及TGF-β1分泌增加-
Abstract:
Objective:To explore the effects of Y-box binding protein 1(YB-1) on the proliferation and transforming growth factor-β1 (TGF-β1) secretion in rat mesangial cells (MC). Methods:Rat mesangial cells were cultured with or without YB-1 over-expression,and the supernatant and the cells were collected respectively. The proliferation of MC with YB-1 over-expression was determined by MTT and cell counting. The level of the TGF-β1 protein in the supernatant was mesured by ELISA,while the TGF-β1 mRNA expression was detected by real-time PCR. Results:YB-1 over-expression stimulated the proliferation of MC,upregulated the expression of TGF-β1 mRNA,and induced the TGF-β1 protein secreting in rat MC. Conclusion:YB-1 triggered MC proliferation and increased TGF-β1 secreting,and inhibiting YB-1 expression may be beneficial in retarding progressive development of glomerulonephritis.
