褪黑素对人胃癌细胞SGC-7901黏附和迁移的影响
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国家自然科学基金青年基金资助项目(81201614);江苏省六大人才高峰 (JY-020)


Melatonin inhibits adhesion and migration of SGC-7901 gastric cancer cells
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    目的:研究褪黑素对人胃癌细胞SGC-7901黏附和迁移能力的影响及探索可能的分子机制-方法:用不同浓度的褪黑素孵育人胃癌细胞SGC-7901 24 h,通过免疫印迹方法检测F-actin和G-actin蛋白表达水平,通过免疫荧光法检测细胞骨架结构的改变,通过细胞黏附实验观察胃癌细胞对血管内皮细胞黏附能力的变化,通过划痕实验检测褪黑素对细胞迁移能力的影响-结果:褪黑素使人胃癌细胞SGC-7901 F-actin量明显降低,同时细胞骨架结构发生重组,应力纤维出现断裂;褪黑素可抑制胃癌细胞的迁移及对血管内皮细胞的黏附能力-结论:褪黑素可能通过破坏细胞内应力纤维结构的稳定,最终导致了胃癌细胞的黏附和迁移能力的降低-

    Abstract:

    Objective:To investigate the effects of melatonin on adhesion and migration abilities of human SGC-7901 gastric cancer cells and its role in reorganization of actin cytoskeleton. Methods:SGC-7901 cells were treated with different concentrations of melatonin for 24h. The contents of G-actin(in cytosolic fraction)and F-actin(in cytoskeletal fraction)in cells were measured by Western blot. Cellular F-actin stained with FITC-phalloidin was examined by fluorescence microscopy. Adhesive assay was used to detect the adhesive ability of SGC-7901 cells to HUVECs. Migration rate was measured by wound healing assay. Results:After treated with melatonin for 24h,the celluar F-actin was depolymerized and actin cytoskeleton was rearranged. Consistently,the abilities of adhesion and migration in SGC-7901 cells were significantly reduced compared to the control cells. Conclusion:Melatonin inhibits the abilities of adhesion and migration in SGC-7901 cells,which may be via reorganization of cellular actin cytoskeleton.

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陈宜刚,许 蕊,杨 玲,崔 洁,杜 军.褪黑素对人胃癌细胞SGC-7901黏附和迁移的影响[J].南京医科大学学报(自然科学版),2014,(12):1644-1648

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  • 收稿日期:2014-06-19
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  • 在线发布日期: 2014-12-26
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