厄洛替尼对非小细胞肺癌增殖和自噬的影响
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苏州市科技发展计划项目(SYSD2012087);苏州大学附属第二医院博士?留学归国人员预研项目(SDFEYBS1102)


Effects of erlotinib on proliferation and autophagy in non-small cell lung cells
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    摘要:

    目的:探讨厄洛替尼对人非小细胞肺癌细胞HCC827-A549-H1299增殖及自噬的影响。方法:将体外培养的人肺癌HCC827-A549-H1299细胞株分为对照组(CON组)-溶剂对照组(DMSO组)和厄洛替尼处理组,采用CCK-8法检测厄洛替尼对细胞增殖的抑制作用,采用Western blot及细胞免疫荧光法检测自噬相关蛋白LC3-Beclin-1表达。结果:厄洛替尼以剂量依赖的方式抑制HCC827-A549-H1299细胞增殖,增殖抑制率由高到低分别为HCC827-A549-H1299,3株细胞的半数抑制浓度分别为8.72-32.09-87.46 μmol/L;与对照组相比,厄洛替尼显著上调LC3-Ⅱ及Beclin-1表达 (P < 0.05);厄洛替尼诱导HCC827-A549-H1299细胞株自噬,且其诱导作用呈剂量依赖性(P < 0.05)。结论:厄洛替尼选择性抑制非小细胞肺癌增殖,并诱导非小细胞肺癌细胞自噬。

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    Objective:To investigate the effects of erlotinib on proliferation and autophagy in HCC827,A549 and H1299 human non-small cell lung cancer cells. Methods:Three human non-small cell lung cancer cell lines HCC827,A549,H1299 were cultured in vitro,and were divided into control group,DMSO group,and groups treated by erlotinib in different concentrations. CCK-8 method was used for detecting the proliferation inhibition. The expression levels of autophagy-related proteins,microtubule-associated protein light chain (LC3)and Beclin-1 Were detected by Western blot analysis and cell immunofluorescence method. Results:Erlotinib inhibited the proliferation of HCC827,A549 and H1299 cells,proliferation inhibition rate showed that HCC827> A549 >H1299,IC50 of HCC827,A549 and H1299 cells were 8.72 μmol/L,32.09 μmol/L and 87.46 μmol/L,respectively. Compared with the control groups,erlotinib enhanced expression of LC3-II and Beclin-1 significantly(P < 0.05). Erlotinib induced autophagy in a concentration dependent manner in HCC827,A549 and H1299 cells(P < 0.05). Conclusion: Erlotinib inhibited proliferation selectively and activated autophagy in non-small cell lung cancer cells.

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杜紫燕,王淅凤,李凌云,嵇成红,施敏骅,余 勇.厄洛替尼对非小细胞肺癌增殖和自噬的影响[J].南京医科大学学报(自然科学版),2015,(5):662-665,690

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  • 收稿日期:2014-12-19
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  • 在线发布日期: 2015-05-22
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