Objective:To analyze the changes of serum molecule metabolites between rats with diabetic gastroparesis and healthy rats. Methods: Partial least squares linear discriminant analysis (PLS-DA) and orthogonal partial least squares linear discriminant analysis (OPLS-DA) were used to identify the differences of two groups on the basis of high performance liquid chromatography. Results: There were statistically significant differences in the amino acids between serum of the two groups, and compared with the normal control group, the contents of threonine, lactamine, aminoacetic acid, creatine and phenylalanine in the diabetic gastroparesis group were significantly decreased, however, the content of .trioxypurine was significantly increased. Conclusion: The results demonstrated that the different amino acids,which included glycocine/creatine/phenylalanine and so on,may be related to the pathogenesis of diabetic gastroparesis. Therefore, it has important forecasting significance to make use of gas chromatographic mass spectrometric analysis to study the pathogenesis of diabetic gastroparesis.