Sonic Hedgehog信号在小脑早期发育中的作用
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国家自然科学基金面上项目(81171962)


The role of Sonic Hedgehog signaling pathway in the early development of the cerebellum
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    目的:研究Sonic Hedgehog(Shh)信号在小脑发育早期对小脑外颗粒层的颗粒细胞前体(GCP)细胞增殖迁移的影响-方法:通过石蜡切片免疫荧光观察小脑外颗粒层GCP细胞增殖情况;建立体外培养小鼠脑片系统,检测小脑外颗粒层GCP细胞的增殖和动态迁移情况-结果:小鼠出生后7 d小脑外颗粒层增殖的GCP细胞最多;Shh信号通路促进小脑外颗粒层GCP细胞的增殖,用环巴胺(cyclopamine,CPA)抑制信号通路后小脑外颗粒层GCP细胞增殖受到抑制,但迁移不受影响-结论:Shh信号通路是促进小脑外颗粒层细胞增殖及有丝分裂必不可少的一部分,且它的作用只部分局限于外颗粒层-

    Abstract:

    Objective:To research Sonic Hedgehog (Shh) signaling pathway effects on the cerebellum external granular layer (EGL layer) cell granule cell precursors (GCP cell) proliferation and migration in early development of cerebellum. Methods: Paraffin sections by immunofluorescence were performed to observe external granular layer of the cerebellum GCP cell proliferation. Mouse brain slices in vitro establish systems were performed to detect dynamic migration and proliferation of cerebellar external granular layer of GCP cells. Results: The number of granulosa cell proliferation precursors was reached the peak on the seventh days after birth in mice cerebellar external granular layer;Shh signaling pathway promoted proliferation of cerebellar external granular layer cells. After inhibition of the signaling pathway by cyclopamine (CPA), cell proliferation of cerebellar external granular layer was inhibited, but migration was unaffected. Conclusion: Shh signaling pathway is a part of promoting cell proliferation of cerebellar external granule layer and mitosis essential, and its function is only limited to the external granule layer.

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刘晓同,李三恩,乐 珅,程 雁. Sonic Hedgehog信号在小脑早期发育中的作用[J].南京医科大学学报(自然科学版),2015,(7):899-904

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  • 收稿日期:2015-03-10
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  • 在线发布日期: 2015-07-12
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