Abstract:Objective:To investigate browning ability differences of adipose tissues in each part of mice (male C57BL/6J). Methods: We isolated adipose tissues from mice (male C57BL/6J). The expressions of browning, adipogenesis, adipokine and inflammation markers were examined by fluorescence quantitative PCR. The protein expression of uncoupling protein-1 (Ucp1) was detected by Western blotting. Results: The expression of browning genes in adipose tissues around the thyroid gland was the highest compared with those in the classic brown adipose tissues (interscapular and infrascapular brown adipose tissues). In mesenteric adipose tissues, perigonadal adipose tissues, and inguinal subcutaneous adipose tissues, browning genes showed the lowest expression. The expression of adipogenesis genes in subcutaneous adipose tissues was significantly higher than those in epididymis and mesenteric adipose tissues. The expression of adipocytokines in white adipocytes was significantly higher than that in brown adipocytes. The expression of inflammatory factor was significantly increased in perigonadal and mesenteric adipose tissues, while it was the lowest in subcutaneous adipose tissues. Conclusion: The browning ability in adipose tissues around the thyroid gland is close to that in interscapular and infrascapular brown adipose tissues. Adipose tissues around the thyroid gland, interscapular subcutaneous adipose tissues and adipose tissues from perirenal and axilla show brown-like abilities just like iBAT. Mesenteric adipose tissues, perigonadal adipose tissues, and inguinal subcutaneous adipose tissues showed strong abilities of adipogenesis and immune. The cytokine expression of mesenteric adipose tissues, perigonadal adipose tissues is lower, but inflammatory factor secretion is relatively higher, and lipolytic effect is stronger.