miR-145在非小细胞肺癌中的表达及其对N-cadherin表达的调控
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江苏省实验诊断学重点实验室(XK201114)


Expression of miR-145 in non-small cell lung cancer and regulatory effect on N-cadherin
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    摘要:

    目的:检测miR-145在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,并探讨其调控神经性钙黏附蛋白(neural cadherin,N-cadherin)在NSCLC侵袭转移中的作用-方法:实时荧光定量PCR检测31例NSCLC组织中miR-145与N-cadherin的表达水平-采用脂质体法将miR-145 mimics瞬时转染至SPC-A1细胞,RT-PCR和Western blot检测N-cadherin的表达变化;Transwell实验检测转染后SPC-A1细胞的侵袭能力-结果:在NSCLC癌组织中,miR-145与N-cadherin的表达水平分别表现为显著下调和上调,且表达水平与淋巴结转移密切相关-在SPC-A1细胞中,过表达miR-145能降低N-cadherin的表达,并抑制肿瘤细胞的侵袭-结论:miR-145在NSCLC中低表达,其可能通过靶向调控N-cadherin而影响NSCLC的侵袭转移-

    Abstract:

    Objective:To detect the expression of miR-145 in non-small cell lung cancer(NSCLC) and explore it’s regulatory effect on N-cadherin in the invasion and metastasis of NSCLC. Methods:Real-Time PCR was performed to detect the expression of miR-145 and N-cadherin in 31 NSCLC tumor tissues. MiR-145 mimics was transiently transfected into SPC-A1 cells by lipofectamine method. RT-PCR and Western blot were used to detect the expression of N-cadherin. The invasion activity was detected using transwell assay. Results:MiR-145 expression was reduced significantly and N-cadherin expression was increased significantly in NSCLC tumor tissues,respectively. In addition,the expressions of miR-145 and N-cadherin were both closely correlated with lymph node metastasis. MiR-145 could significantly downregulate the expression of N-cadherin after transfection of mimics in SPC-A1 cells,and suppress invasion ability of cells. Conclusion:MiR-145 is downregulated in NSCLC,and may regulate the invasion and metastasis of NSCLC through targeting N-cadherin.

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莫冬萍,龚 雪,黄珮珺,许雨乔,黄 蕾,潘世扬,徐 建. miR-145在非小细胞肺癌中的表达及其对N-cadherin表达的调控[J].南京医科大学学报(自然科学版),2015,(7):945-949

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  • 收稿日期:2015-01-13
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  • 在线发布日期: 2015-07-12
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