肾母细胞瘤过表达基因促进肝癌细胞的侵袭与转移
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

卫生部医药发展项目(W2012Fz058)


Nephroblastoma overexpressed gene promotes migration and invasion of hepatocellular carcinoma
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨肾母细胞瘤过表达基因(NOV CCN3)在肝癌中的表达及在肝癌侵袭中的作用。方法:荧光定量PCR(real-time PCR)检测肝癌及癌旁组织中的CCN3的mRNA表达水平,同时检测肝癌细胞系及正常人肝细胞中CCN3的mRNA表达水平,Western blot检测肝癌及癌旁组织中CCN3蛋白的表达量;通过肝癌细胞过表达CCN3分析CCN3蛋白对肝癌细胞转移的作用;运用Transwell侵袭实验和划痕迁移实验研究CCN3过表达对肝癌细胞侵袭和迁移能力的影响。结果:50对样本中有39对CCN3的mRNA表达水平在肝癌组织中高于癌旁组织,Western blot提示肝癌组织中CCN3蛋白表达量高于癌旁组织。体外肝癌细胞的功能试验中,过表达CCN3能促进MHCC-97H-SMMC-7721细胞的侵袭与转移。结论:CCN3在肝癌组织中高表达,过表达CCN3能促进肝癌的侵袭与转移,CCN3发挥作用机制的研究能为肝细胞肝癌的治疗提供新的思路。

    Abstract:

    Objective:To detect the expression and study the role of nephroblastoma overexpressed gene (NOV CCN3)in hepatocelluar carcinoma(HCC). Methods:The expressions of CCN3 mRNA in HCC samples and cell lines were detected by real-time PCR,and the expressions of CCN3 protein in HCC tissues and adjacent non-cancer tissues were assessed by Western blot. In addition,invasion of HCC cells was observed after overexpressing CCN3 by Transwell invasion assay and wound healing assay. Results:In the total of 50 paired HCC specimens,compared with the adjacent non-cancer tissues,the expression of CCN3 mRNA was up-regulated in 39 cases,and the expression of CCN3 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of CCN3 enhanced HCC-derived MHCC-97H,SMMC-7721 cellular invasion in vitro. Conclusion:This study indicates CCN3 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. The study of CCN3 may provide a novel therapeutic idea for prevention and treatment of invasion in HCC.

    参考文献
    相似文献
    引证文献
引用本文

卢叶挺,丁文周,于建军,谭龙威,孔连宝.肾母细胞瘤过表达基因促进肝癌细胞的侵袭与转移[J].南京医科大学学报(自然科学版),2015,(8):1087-1091

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2015-03-13
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-08-04
  • 出版日期:
通知关闭
郑重声明