Objective:To identify the virus strain isolated from a suspected dengue case imported to Jiangsu province from Philippines in April 2012 and analyze its molecular characteristics. Methods:The serum sample collected from the patient was detected for IgM and IgG by colloidal gold method. The sample was further inoculated in C6/36 cells for virus isolation. Dengue nucleic acid was detected in the isolates via a stable cytopathic effect on C6/36 cells according to reverse translation-PCR and real-time PCR. Genome sequence information from the isolated strain was obtained by next-generation high-throughput sequencing. Nucleotide alignment and phylogenetic tree construction were conducted by MEGA6.0 software. Results:IgM and RNA of type 1 dengue virus were detected in the serum sample. The nucleotide homology with dengue virus 1 isolated from American Haw O3663 was more than 98%. Conclusion:The imported case of fever was caused by the dengue virus type 1.