Abstract:Objective:To observe the effects of TLR7 agonist on the immune function of IL-10 transfected bone-marrow derived dendritic cell(mDC). Methods:The IL-10 plasmid was transfected to mouse mDC,and TLR7 agonist,Imiquimod,was added to mDC for 48 h. The expression of MHCⅡ,CD80,CD86,FasL in mDC were detected with flow cytometry,and the production of the IL-6 and TNF-α was detemined by ELISA. Results:IL-10 suppressed the expression of MHCⅡ,CD80,CD86,decreased the production of IL-6 and TNF-α,and increased the expression of FasL on mDC. Imiquimod increased the expression of MHCⅡ,CD80 and CD86,which enhanced the production of IL-6 and TNF-α,but inhibited of FasL expression by the IL-10 transfected mDC. Conclusion:TLR7 activation reverses IL-10 induced low response of mDC.
