锌对2型糖尿病大鼠糖脂代谢的影响
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江苏省高校优势学科建设工程项目(YSHL0301-01)


Zinc supplementation improves glucose and lipid metabolism in type 2 diabetic rats
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    摘要:

    目的:观察补锌对2型糖尿病大鼠糖-脂代谢的影响,并探讨作用机制。方法:采用高糖高脂饲料喂养8周后,腹腔注射30 mg/kg链脲佐菌素(STZ)制备2型糖尿病大鼠模型。将造模成功的大鼠随机分为模型组和硫酸锌低-中-高剂量组,另设同龄大鼠为正常对照组。硫酸锌组按照低剂量[1.6 mg/(kg·d)]-中剂量[3.2 mg/(kg·d)]-高剂量[6.4 mg/(kg·d)]每日灌胃补锌,模型组与正常对照组仅给予等量纯净水。灌胃6周后检测大鼠血清锌-糖-脂代谢-氧化应激和炎症的相关指标,包括空腹血糖(FBG)-空腹胰岛素(FINS)-胰岛素抵抗指数(HOMA-IR)-血清总胆固醇(TC)-甘油三酯(TG)-高密度脂蛋白胆固醇(HDL-C)-低密度脂蛋白胆固醇(LDL-C)-游离脂肪酸(FFA)-超氧化物歧化酶(SOD)-谷胱甘肽过氧化物酶(GSH-Px)-丙二醛(MDA)-肿瘤坏死因子(TNF)-α及白细胞介素(IL)-6。结果:补锌可以显著升高2型糖尿病大鼠血清锌水平,降低FBG-FINS-HOMA-IR及血清TC-TG-LDL-C-FFA水平,升高血清HDL-C浓度,增加抗氧化酶的活性,减少脂质过氧化物和炎症因子的产生,且以高剂量补锌组效果最佳。结论:补锌可以改善STZ诱导的2型糖尿病大鼠糖-脂代谢异常,可能与锌的抗氧化和抗炎作用机制有关。

    Abstract:

    Objective:This study was conducted to explore whether zinc supplementation improves the disturbance of glucose and lipid metabolism in type 2 diabetes mellitus (T2DM) rats and how did these produce. Methods: T2DM rat model was established by feeding with high-sucrose-fat diet for 8 weeks followed by streptozotocin (STZ) (30 mg/kg, i.p). T2DM rats were randomly divided into four groups. The zinc supplementation groups were respectively administrated 1.6 mg/kg, 3.2 mg/kg and 6.4 mg/kg elemental zinc as zinc sulfate by gavage, while both the normal control group and untreated diabetic group received ultrapure water. After 6 weeks, we detected the indexes about serum zinc, blood glucose, lipid metabolism, oxidative stress and inflammatory cytokines, which including fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment-insulin resistance (HOMA-IR), serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), free fatty acids (FFA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malonaldehyde (MDA), tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. The results indicated that zinc supplementation significantly increased serum zinc and HDL-C levels and reduced FBG, FINS, HOMA-IR and serum TC, TG, LDL-C, FFA levels. The activities of antioxidant enzymes were significantly enhanced, while inflammatory cytokines were decreased in the zinc supplementation groups. The high dose of the zinc supplementation group (6.4 mg/kg·d) showed the best curative effects. Conclusion: The present study showed that zinc supplementation could improve metabolic disturbance of type 2 diabetic rats, and that could be related to augmenting antioxidant and anti-inflammatory ability.

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陈 璇,周方圆,魏小飞,吴 冰,汪 悦.锌对2型糖尿病大鼠糖脂代谢的影响[J].南京医科大学学报(自然科学版),2015,(10):1377-1382

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  • 收稿日期:2015-06-01
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  • 在线发布日期: 2015-10-28
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