非增殖性糖尿病视网膜病变患者血清miR-29c的表达及相关分析
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国家自然科学基金(81370920)


Expression of serum miR-29c and its correlation analysis in patients with non-proliferative diabetic retinopathy
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    摘要:

    目的:观察微小RNA-29c(miR-29c)在非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)患者血清中的表达水平,探讨其在糖尿病视网膜病变(diabetic retinopathy,DR)发病机制中的作用。方法:选择2型糖尿病NPDR患者及无视网膜病变(non-diabetic retinopathy,NDR)患者各30例。收集血清样本,应用荧光定量PCR方法检测血清miR-29c的相对表达水平。应用PicTar-TargetScan与MiRanda软件综合预测miR-29c的靶基因,取三款软件的交集作为最终靶基因。结果:miR-29c在NPDR患者血清中的表达量明显高于NDR组(P < 0.05),其表达量与HbA1c呈显著正相关(r=0.379,P < 0.05)。预测miR-29c靶基因集合富集在磷酸肌醇代谢-细胞因子及受体的相互作用-细胞外基质与受体信号作用通路中。结论:NPDR患者血清miR-29c表达显著上调,miR-29c可能通过调控细胞因子的相互作用-磷酸肌醇代谢等途径参与DR的发生发展。

    Abstract:

    Objective:To investigate the expression of serum miR-29c in the patients with non-proliferating diabetic retinopathy (NPDR) and explore the role of miR-29c in diabetic retinopathy (DR). Methods: Patients with NPDR (n=30) and without DR (n=30) were enrolled. Total RNAs including miRNA was extracted from serum samples. The levels of miR-29c were detected by quantitative Real-time PCR. PicTar, TargetScan and MiRanda were performed to comprehensively predict target genes of miR-29c, and the intersection of the three softwares was set as the final target genes. Results: The expression of serum miR-29c was significantly elevated in the NPDR group than that in the NDR group (P < 0.05). Positive correlation between miR-29c expression and HbA1c level (r=0.379, P < 0.05) was observed. The target genes of miR-29c were significantly enriched in inositol phosphate metabolism, interaction between cytokine and cytokine receptor, and ECM and receptor. Conclusion: The serum levels of miR-29c were significantly increased in the NPDR group. MiR-29c may play an important role in pathogenesis of DR by regulating cytokines interaction and inositol phosphate metabolism.

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吴心池,王 尧,唐 伟,朱 琳,高 远.非增殖性糖尿病视网膜病变患者血清miR-29c的表达及相关分析[J].南京医科大学学报(自然科学版),2015,(10):1401-1404

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  • 收稿日期:2015-06-06
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  • 在线发布日期: 2015-10-28
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