Objective:To analyze the expression profile variation of circular RNA(circRNA) between normal colorectal tissues and colorectal cancer(CRC) tissues. Methods: Human circRNA microarray was performed to detect the difference of circRNA expression between 6 cases of CRC tissues and 6 cases of normal colorectal tissues. After pretreatment and homogenization of raw data,the circRNAs with differental expression were screened out and then analyzed by hierarchical clustering and scatter volcano diagram. Results: Compared with normal colorectal tissues, there were 892 circRNAs differentially expressed which have more than 2 times variation and significant difference(P < 0.05). Among them, 412 increased and change′s fold more than 2 times,while 480 reduced and change′s fold more than 2 times. Conclusion: The circRNA expression profile of CRC changed significantly in comparison with the normal colorectal tissues, the different circRNAs may be associated with the development of CRC.