白细胞介素6基因启动子-634C/G 多态性与冠心病的关联
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南通市临床医学专项资助(HS2013069)


Association between interleukin-6 promotor-634C/G polymorphism and coronary artery disease
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    摘要:

    目的:探讨白细胞介素(interleukin t,IL)-6基因启动子-634C/G多态性与冠心病的相关性。方法:纳入86 例冠心病及91例对照组患者,检测相关生化指标,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测两组患者IL-6基因型,酶联免疫吸附(ELISA)法检测IL-6血清水平。比较两组间基因型-等位基因频率以及不同基因型对血清IL-6 水平的影响。结果:冠心病组与对照组间年龄-性别-吸烟史-高血压病史及相关生化指标未见统计学差异(P > 0.05),冠心病组外周血IL-6水平明显高于对照组[(26.57 ± 6.68)ng/L vs. (6.89 ± 2.32)ng/L,P < 0.05],IL-6基因启动子-634C/G 3种基因型(CC-CG-GG)在冠心病组中的频率为57%-29%-14%,在对照组中的频率为65.9%-31.9%-2.2%,两组间基因型频率存在统计学差异(P < 0.05)。冠心病组中IL-6基因启动子位点G等位基因携带者(GG-CG基因型)血清IL-6水平高于不携带者[(34.575 ± 6.618)ng/L vs. (16.758 ± 5.182)ng/L,P < 0.05]。结论:IL-6基因启动子634C/G基因多态性与冠心病相关,其中G等位基因携带者外周血IL-6 水平显著增高。

    Abstract:

    Objective: To explore the association of-634C/G polymorphism of interleukin-6 (IL-6) gene with coronary artery disease(CAD). Methods: A total of 86 CAD patients and 91 healthy controls were selected for this study. The PCR-based restriction fragment length polymorphism (PCR-RFLP) technique was performed to assess the genotypes frequencies. The concentration of serum IL-6 was measured with ELISA. The relations of the levels of serum IL-6 between groups were analyzed. Results: The circulating IL-6 levels in the CAD group[(26.57 ± 6.68) ng/L] were significantly higher than those in the control group[(6.89 ± 2.32) ng/L,P < 0.05]. The distribution of the IL-6 -634C/G genotypes (CC,CG, and GG) was 65.9%,3 1.9% and 2.2% in the controls,and 57%, 29% and 14% in the CAD group, respectively(P < 0.05). Compared with the wild type CC,the G allele carriers(CG+GG genotypes) had higher circulating IL-6 levels[(34.575 ± 6.618)ng/L vs.(16.758 ± 5.182)ng/L, P < 0.05] in the CAD group. Conclusion:These findings suggest that IL-6 -634C/G polymorphism is associated with CAD and the G allele carriers have increased circulating IL-6 levels.

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黄 荣,潘 闽,蔡星星,顾周山,苏亚民,朱健华.白细胞介素6基因启动子-634C/G 多态性与冠心病的关联[J].南京医科大学学报(自然科学版),2015,(11):1557-1560

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  • 收稿日期:2015-05-29
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  • 在线发布日期: 2015-11-30
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