miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响
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江苏省六大人才高峰项目(2013)


The effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells
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    摘要:

    目的:探讨miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响。方法:慢病毒法将miRNA145转染入人结直肠癌细胞株HCT116,实时定量PCR检测细胞miRNA145表达的变化。通过CCK-8细胞增殖实验检测miRNA145和/或二甲双胍对HCT116细胞增殖的影响。通过Western blot检测miRNA145和/或二甲双胍对细胞AMPK-mTOR蛋白表达水平的影响。结果:CCK-8实验表明miRNA145-二甲双胍均具有抑制HCT116细胞增殖的作用,二者联用使细胞增殖受到更大程度的抑制。Western blot结果表明,二甲双胍-miRNA145以及二者联用可进一步促进AMPK的活化进而影响mTOR蛋白表达水平。结论:miRNA145联合应用二甲双胍具有更强的抑制人结肠癌细胞株HCT116增殖的作用。

    Abstract:

    Objective:To investigate the effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells. Methods:miRNA145 overexpression mediated by recombinant lentivirus was exployed in HCT116 cells. Cell proliferation assay by CCK-8 method was conducted to determine the roles of miRNA145 and/or metformin on the cancer cells. Western blot analysis was used to determine the expression levels of AMPK and mTOR proteins. Results:Both miRNA 145 and metformin can inhibit the cell proliferation of HCT116. The combination of miRNA 145 and metformin exerted a much stronger inhibitory effect on cell proliferation than any of the two. The activation level of AMPK was also higher by the combination,and the p-mTOR expression level was lower by the combination. Conclusion:miRNA145 and metformin can exert an anticancer effect synergistically.

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王钟林,张冬生,王 勇,孙跃明. miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响[J].南京医科大学学报(自然科学版),2015,(12):1703-1706

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  • 收稿日期:2015-09-12
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  • 在线发布日期: 2016-01-04
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