脓毒症患者外周血调节性Vδ1 T细胞比例及功能变化
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Changes of number and function of peripheral blood Vδ1 T cells in patients with sepsis
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    摘要:

    目的:观察脓毒症患者外周血Vδ1 T细胞数量和功能的改变,初步探讨Vδ1 T细胞在脓毒症发病中的作用-方法:选取2013年12月—2014年12月在本院ICU入住的脓毒症患者40例及同期入院进行健康体检者(health control,HC)40例,清晨空腹抽取外周静脉血10 mL-采用流式细胞术测定其外周血Vδ1 T细胞比例及Vδ1 T细胞表面Foxp3表达情况,采用CFSE染色方法检测Vδ1 T细胞对CD4+ T细胞增殖能力的影响,采用流式细胞术测定Vδ1 T细胞与CD4+ T细胞共孵育后对CD4+ T细胞分泌γ-干扰素(interferon γ,IFN-γ)及肿瘤坏死因子α(tumor necrosis factor α,TNF-α)能力的影响-结果:脓毒症患者外周血Vδ1 T细胞数量(3.35 ± 0.55)%与HC(1.04 ± 0.22)%相比显著升高(P < 0.01)-与HC外周血Vδ1 T细胞表面Foxp3表达水平(10.02 ± 2.31)%相比,脓毒症患者外周血Vδ1 T细胞表面Foxp3表达水平(16.83 ± 3.63)%显著升高(P < 0.01)-HC外周血Vδ1 T细胞对CD4+ T细胞增殖-分泌IFN-γ和TNF-κ的抑制百分率分别为(44.26 ± 6.31)%-(34.84 ± 4.83)%和(39.31 ± 4.91)%,脓毒症患者外周血Vδ1 T细胞对CD4+ T细胞增殖-分泌IFN-γ和TNF-κ的抑制百分率分别为(62.35 ± 5.91)%-(50.37 ± 4.77)%和(54.94 ± 5.71)%-提示,脓毒症外周血Vδ1 T细胞较HC外周血Vδ1 T细胞具有更强的免疫抑制功能(P < 0.01)-结论:脓毒症患者外周血Vδ1 T细胞免疫抑制功能增强,致使脓毒症患者免疫功能受到抑制,脓毒症患者免疫功能的这种改变可能与脓毒症的发生相关-

    Abstract:

    Objective:To observe changes of the number and function of peripheral blood Vδ1 T cells in patients with sepsis, and preliminary study on the role of Vδ1 T cells in the pathogenesis of sepsis. Methods: A total of 40 patients with sepsis who underwent ICU treatment and 40 healthy controls (HC)who underwent physical examination in authors’ affiliated hospital from December, 2012 to December, 2014 were enrolled in this study. Peripheral venous blood samples (10 mL) were taken from both groups. The proportion of Vδ1 T cells and Foxp3 expression on Vδ1 T cells were detected by flow cytometry (FCM). The inhibition effect of Vδ1 T cells on CD4+ T cell proliferation was detected by CFSE staining. After coculture for 72 h, the inhibition effects of Vδ1 T cells on the IFN-γand TNF-α production ability of CD4+ T cell were detected by FCM. Results: The proportion of Vδ1 T cells in HC was(1.04 ± 0.22)%, and in sepsis was (3.35 ± 0.55)%, the proportion of Vδ1 T cells between HC and sepsis had statistical significance (P < 0.01). The proportion of Foxp3 expression on Vδ1 T was (10.02 ± 2.31) % in HC, and was (16.83 ± 3.63)% in sepsis, the proportion of Foxp3 expression on Vδ1 T between HC and sepsis had a statistical significance (P < 0.01). The inhibitory effect of Vδ1 T cells from HC on the proliferation, IFN-γ and TNF-α production ability of CD4 T cell were (44.26 ± 6.31)%,(34.84 ± 4.83)% and (39.31 ± 4.91)%, respectively; and the inhibitory effect of Vδ1 T cells from sepsis on the proliferation, IFN-γand TNF-α production ability of CD4 T cell were (62.35 ± 5.91)%,(50.37 ± 4.77)% and(54.94 ± 5.71)%, respectively. The inhibitory effect of Vδ1 T cells between HC and sepsis had a statistical significance (P < 0.01). Conclusion: Vδ1 T cells from sepsis have strong inhibitory function, resulting in immune function inhibition, finally resulting in sepsis.

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谢 谦,周 丽.脓毒症患者外周血调节性Vδ1 T细胞比例及功能变化[J].南京医科大学学报(自然科学版),2016,(1):73-76

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  • 收稿日期:2015-06-20
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  • 在线发布日期: 2016-01-30
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