miR-196a2 T>C基因多态与宫颈癌易感性的关系研究
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Polymorphism of the pre-mir-196a2 is associated with susceptibility of cervical cancer in Jiangsu population
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    摘要:

    目的:探讨miR-196a2 T>C基因多态与江苏人群宫颈癌遗传易感性的关系-方法:选择经组织学确诊的509例新发宫颈癌患者作为病例组,与病例组人群不存在生物学相关的562例正常人群作为对照组,利用Taqman实时荧光定量PCR技术检测基因型;选择宫颈鳞状细胞癌标本20例,实时荧光定量PCR(RT-qPCR)检测各基因型miR-196a的表达量;Logistic回归模型计算基因型与人群罹患宫颈癌的风险比(odds ratio,OR)及其95%的可信区间(95%CI)-结果:携带miR-196a2突变纯合子CC个体罹患宫颈癌的风险明显高于TT基因型个体(OR=1.45,95%CI:1.04~2.09),携带TC/CC基因型个体增加约33%的宫颈癌发病风险(OR = 1.33, 95%CI:1.06~1.81);对宫颈鳞状细胞癌(cervical squamous cell carcinoma,CSCC)分析,与TT基因型比较,含突变纯合子CC个体罹患CSCC的风险明显升高(OR=1.49,95%CI:1.05~2.11),携带TC/CC基因型个体增加约31%的CSCC发病风险(OR = 1.31,95%CI:1.00~1.73);RT-qPCR显示,在宫颈鳞癌组织中CC及CT/CC基因型与TT基因型相比,成熟miR-196a的表达水平明显升高-结论:MiR-196a2 T>C多态变异与中国江苏人群宫颈癌遗传易感性关联密切-

    Abstract:

    Objective:To study the possible association between miR-196a2 gene polymorphisms and cervical cancer susceptibility among women in Jiangsu Province. Methods:The subjects included 509 cervical cancer cases and 562 cancer-free controls. We genotyped the functional polymorphism of miR-196a2 (rs11614913)by TaqMan-Probe assay method,and used a sample of 20 cervical cancer tissues to test the expression of mature miR-196a by real-time quantitative reverse transcription. Results:When compared with TT genotype,the variant homozygote CC genotype were associated with increased cervical cancer risk(OR=1.45,95%CI = 1.04~2.09) and cervical squamous cell carcinoma(CSCC) risk(OR=1.49,95%CI = 1.05~2.11); moreover,CC genotype was associated with high miRNA levels in CSCC tissues,whereas TT genotype showed a relatively low expression in comparison to both TC/CC (P=0.040) and CC patients(P=0.030). Conclusion:The natural genetic variation in pre-miR-196a2 may affect the amount of mature miR-196a in vivo,and contribute to the susceptibility to cervical cancer.

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丁 波,王美林,张正东,韩素萍. miR-196a2 T>C基因多态与宫颈癌易感性的关系研究[J].南京医科大学学报(自然科学版),2016,(1):85-89

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  • 收稿日期:2015-08-12
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  • 在线发布日期: 2016-01-30
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