c-Myc基因在人非小细胞肺癌中的表达及基因沉默抑制H1299细胞效果的实验研究
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江苏省六大人才高峰(2012-WS-108)


The expression of c-Myc in non-small cell lung cancer and the effect of its silencing in inhibiting tumor cells
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    摘要:

    目的:分析人非小细胞肺癌(non-small cell carcinoma,NSCLC)组织中c-Myc基因的表达,探讨沉默c-Myc基因在非小细胞肺癌基因治疗中的前景-方法:应用实时荧光定量PCR法检测84例非小细胞肺癌组织及其相应癌旁组织中c-Myc基因mRNA的表达情况-通过脂质体LipofactAMINE2000将c-Myc基因siRNA瞬时转染至非小细胞肺癌H1299癌株细胞中,顺铂(Cisplatin,cis)药物毒性实验验证c-Myc基因沉默对肿瘤细胞的抑制效果-应用RT-PCR检测H1299细胞中c-Myc基因RNA的表达,CCK-8法检测抑制细胞的效果-结果:非小细胞肺癌组织中c-Myc基因mRNA的表达水平高于相应的癌旁组织,差异有统计学意义(P < 0.05)-c-Myc siRNA瞬时转染后的H1299细胞中,c-Myc基因mRNA的表达水平显著下降(P < 0.05)-将c-Myc基因沉默和顺铂联合使用,还可以显著提高顺铂对H1299细胞增殖的抑制效果(P < 0.05)-结论:c-Myc基因在非小细胞肺癌组织中的表达水平明显高于相应癌旁组织,沉默c-Myc基因能显著提高顺铂抑制非小细胞肺癌H1299 细胞增殖的作用,c-Myc基因有可能成为NSCLC基因治疗的新靶点-

    Abstract:

    Objective:To investigate the expression of c-Myc in non-small cell lung cancer (NSCLC)tissues,and to explore the prospects of the c-Myc gene silencing in NSCLC gene therapy. Methods:The expression levels of c-Myc mRNA in 84 NSCLC tissues and the corresponding para-cancerous tissues were detected by RFQ-PCR(real-time fluorescence quantitative-PCR). C-Myc siRNA was transfected into NSCLC H1299 cells by LipofectAMINE 2000. Cisplatin toxicity experiments further validated effect of inhibiting tumor cells with c-Myc gene silencing. The expression level of c-Myc mRNA in H1299 cells was detected by RT-PCR,and the effect of inhibiting H1299 cells was detected by CCK-8 (Cell Counting Kit-8). Results:The expression level of c-Myc mRNA in NSCLC tissues was higher than that in the corresponding para-cancerous tissues (P < 0.05). C-Myc siRNA was successfully transfected into NSCLC H1299 cells. After transfection,the expression level of c-Myc mRNA was down-regulated in H1299 cells. Effect of inhibiting tumor cells increased significantly when the c-Myc gene silencing and cisplatin were in combination (P < 0.05). Conclusion:The expression level of c-Myc mRNA in NSCLC tissues is higher than that in the corresponding para-cancerous tissues. The lower expression of c-Myc can significantly inhibit NSCLC H1299 cells. It is suggested that c-Myc may act as an oncogene in NSCLC gene therapy.

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王亚勤,刘 翔,吴 晨,唐义虎,吴延虎. c-Myc基因在人非小细胞肺癌中的表达及基因沉默抑制H1299细胞效果的实验研究[J].南京医科大学学报(自然科学版),2016,(2):151-154

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  • 收稿日期:2015-06-13
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