高良姜素对臭氧所致COPD气道炎症的影响
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江苏省科技创新与成果转化(生命健康科技)专项项目(BL2012012)


Effect of galangin on ozone exposure-induced airway inflammation of COPD
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    目的:研究高良姜素对臭氧所致慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)小鼠气道炎症的影响并探讨其可能机制-方法:32只C57BL/6雄性小鼠随机分为正常对照组-COPD组-COPD+高良姜素组-高良姜素单独给药组-COPD组和COPD+高良姜素组反复给予臭氧吸入,每周3次-1次3 h,共12周;对照组吸入空气-取肺组织切片行苏木素-伊红(hematoxylin-eosin,HE)染色观察气管旁及血管旁炎性细胞浸润;收集支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF),并用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测BALF中炎症因子水平;实时荧光定量(reverse transcription-polymerase chain reaction,RT-PCR)检测肺组织核转录因子E2相关因子2(nuclear factor-erythroid related factor 2,Nrf-2)及Keap1(Kelch-like ECH-associated protein1,Keap1)mRNA的表达-结果:与正常对照组相比,COPD模型组小鼠气道内炎症细胞浸润程度明显,BALF中炎性细胞亦明显增多;BALF中白介素(interleukin,IL)-6-IL-8及肿瘤坏死因子(tumor necrosis factor,TNF)-α水平显著增高(P < 0.05);给予高良姜素可显著缓解炎症严重程度并抑制IL-6-IL-8及TNF-α分泌(P < 0.05)-同时高良姜素可使COPD组肺组织Nrf-2水平增高而Keap1未见明显变化-结论:高良姜素可以有效抑制COPD小鼠气道炎症的发生和进展,其机制可能与Nrf-2-Keap1抗氧化系统有关-

    Abstract:

    Objective:To study the effect of galangin on ozone exposure-induced airway inflammation of chronic obstructive pulmonary disease (COPD) in mice and to explore its possible mechanism. Methods:A total of 32 male C57BL/6 mice were randomly divided into four classes:the control group,COPD group,COPD+galangin group and galangin group. They were exposed to ozone for 3 h per day,twice a week for a period of 12 weeks. The control mice were exposed to normal air. Lung sections were stained with hematoxylin-eosin (HE) to assess inflammatory cell infiltration. Bronchoalveolar lavage fluid (BALF) was collected to detect cell counts and cytokines level by enzyme linked immunosorbent test (ELISA). Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to assess the expression of nuclear factor-erythroid related factor 2 (Nrf-2) mRNA and Kelch-like ECH-associated protein1 (Keap1) mRNA. Results:Airway inflammatory cell infiltration in the COPD group significantly increased,and inflammatory cells,interleukin (IL-) 6,IL-8 and TNF-α in the BALF were also notably increased in the COPD group compared with the control group (P < 0.05). Galangin significantly alleviated the severity of airway inflammation and inhibited IL-6,IL-8 and TNF-α secretion (P < 0.05). At the same time,galangin significantly increased Nrf-2 mRNA expression,while Keap1 mRNA had no significant change. Conclusion:Galangin can effectively inhibit the occurrence and progression of COPD airway inflammation,possibly due to the Nrf2-keap1 antioxidant system.

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王晓月,葛 爱,孙培莉,曾晓宁,黄 茂.高良姜素对臭氧所致COPD气道炎症的影响[J].南京医科大学学报(自然科学版),2016,(6):700-704

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  • 收稿日期:2015-10-14
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  • 在线发布日期: 2016-06-15
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