白介素-11在小鼠肝脏缺血再灌注中的作用
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国家自然科学基金(81100318,81270483)


Effects of interleukin-11 (IL-11) on mouse liver warm ischemia/reperfusion (WI/Rp) injury
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    摘要:

    目的:探讨白介素-11(interleukin-11,IL-11)在小鼠肝脏缺血再灌注中的作用。方法:将20只健康雄性C57BL/6小鼠随机分成正常对照组-假手术组-缺血再灌注组和IL-11处理组,各组5只。采用70%肝脏缺血再灌注模型(缺血1 h,再灌注6 h)。缺血再灌注组及IL-11处理组分别于术前2 h给予PBS及IL-11尾静脉注射。通过酶联免疫吸附试验(ELISA法)分别测定血清中丙氨酸氨基转移酶(ALT)-天门冬氨酸氨基转移酶(AST)-白介素-6(IL-6)-白介素-1β(IL-1β)-肿瘤坏死因子-α(TNF-α)水平。通过光镜观察组织学苏木精-伊红(HE)染色改变。应用反转录酶-聚合酶链反应(RT-PCR)检测肝组织IL-6-IL-1β-TNF-α水平。结果:缺血再灌注组及IL-11处理组肝酶水平明显高于正常对照组及假手术组(P < 0.05)。同时IL-11处理组又明显低于缺血再灌注组(P < 0.01)。缺血再灌注组镜下可见大面积肝细胞水肿及少量嗜酸性变,而IL-11处理组肝细胞水肿明显少于缺血再灌注组。IL-11处理组血清及肝组织中IL-6-IL-1β-TNF-α表达水平明显低于缺血再灌注组(P < 0.05)。结论:IL-11预处理可以通抑制IL-6-IL-1β-TNF-α的表达来减轻小鼠肝脏的缺血再灌注损伤。

    Abstract:

    Objective:To investigate the effects of IL-11 on mouse liver warm ischemia/reperfusion (WI/Rp) injury. Methods: A total of 20 healthy male C57BL/6 mice, weighing (22 ± 3) g, were randomly divided into four main experimental groups (n=5 each), including normal group, sham group, ischemia/reperfusion (I/R) group, and IL-11 pretreatment group. We chose a nonfatal model of 70% liver WI/Rp (treated with 1 h ischemia ,and then 6 h reperfusion). The mice of I/R group were injected with PBS, and IL-11 pretreatment group with IL-11, at 2 h before operation. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) in serum were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-6, IL-1β and TNF-α were examined by reverse transcription-polymerase chain reaction (RT-PCR). Histological haema (HE) stained sections were histopathologically examined using light microscopy. Results: The liver enzyme levels were significantly increased in the I/R and IL-11 pretreatment group, compared to those in the normal and sham group (P < 0.05). Meanwhile, the transaminase levels in the IL-11 pretreatment group were significantly reduced compared to those in the I/R group (ALT:P=0.003,and AST:P < 0.001, respectively). Liver cell hydropic and acidophilic degeneration were increased in the I/R and IL-11 pretreatment group, compared to those in the normal and sham group. Besides, the cell hydropic and acidophilic degeneration in the IL-11 pretreatment group was significantly reduced, compared to that in the I/R group. The expressions of IL-6, IL-1β and TNF-α of blood serum and liver tissue were reduced in the IL-11 pretreatment group, compared to those in the I/R group (P=0.0025, P=0.0159, P=0.0175, P=0.0076, P=0.0005, and P=0.0004, respectively). Conclusions: Pretreatment with IL-11 protects mouse livers from WI/Rp injury by suppressing the expressions of IL-6, IL-1β and TNF-α.

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冯之文,于建军,谭龙威,孔连宝.白介素-11在小鼠肝脏缺血再灌注中的作用[J].南京医科大学学报(自然科学版),2016,(7):802-805

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  • 收稿日期:2015-11-12
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  • 在线发布日期: 2016-07-15
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