Objective:To study the effects of glibenclamide on long-term neurological deficits of cerebral ischemia/reperfusion injury in mice. Methods:C57BL/6 mice were performed to establish the cerebral ischemia/reperfusion injury models by occluding the right middle cerebral artery (MCAo). Then,we continuously treated them with glibenclamide (20 mg/kg)by intragastric administration for 5 weeks. We monitored the fasting blood sugar (FBS)and weight of mice per week. We observed the neurobehavioral changes by behavior tests including corner test,cylinder test,rotarod test and foot fault test. Moreover,we observed the changes of astrocytes by immunohistochemistry staining. Results:Continuous treatment with glibenclamide for 5 weeks had no effect on the FBS. Compared to the control group,it significantly increased the body weight at the 2nd week of treatment(P < 0.01),and at the 3rd week of treatment,it reduced the foot fault rate (P < 0.05)and prolonged the time of animals remained on the rotating rod (P < 0.05). In addition,it reduced the area of glial scar (P < 0.05). Conclusion:Continuous treatment with glibenclamide can advance long-term functional recovery of cerebral ischemia/reperfusion injury in mice.