小鼠腹腔巨噬细胞对调节性T细胞体外诱导的影响
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国家高科技研究发展计划(2015AA020932);国家自然科学基金(81100270,81571564);江苏省自然科学基金(BK20131024);江苏省六大人才高峰项目(2011-ws-106);江苏省优秀医学学术重点项目(RC2016)


Effect of peritoneal macrophages on regulating the induction of murine regulatory T cells
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    摘要:

    目的:探讨不同亚型的腹腔巨噬细胞对调节性T细胞(regulatory T cell,Treg)体外诱导过程的影响及可能机制。方法:按Treg的不同诱导条件,分为不加巨噬细胞的正常Treg诱导组(noM组)-原代巨噬细胞影响组(M0组)-M1型巨噬细胞影响组(M1组)-M2型巨噬细胞影响组(M2组)。采用流式细胞术检测不同诱导条件下Foxp3表达以及效应性T细胞增殖情况,HE染色检测不同条件诱导的Treg处理肝脏缺血再灌注模型小鼠的肝脏病理,全自动生化分析仪分析各组小鼠血清ALT水平。结果:与noM组相比,M0组与M2组诱导Foxp3表达明显降低(P < 0.01),而M1组对Fxop3诱导无明显影响。此外,M0与M2组Treg体外抑制功能以及体内保护功能明显低于noM和M1组(P < 0.01),而这种作用的实现依赖细胞之间的接触性作用(P < 0.05)。结论:腹腔巨噬细胞能够影响Treg的体外诱导,不同亚型腹腔巨噬细胞对Treg影响不同,共同为机体免疫平衡发挥着关键性作用。

    Abstract:

    Objective:To investigate the effect and mechanism of different macrophage subtypes on the induction process of regulatory T cells(Tregs). Methods: The cells was divided into the normal group(noM group),the primary macrophages effect group (M0 group),the M1 effect group (M1 group) and the M2 effect group (M2 group) according to different induction conditions. Foxp3 expression of Tregs and proliferation of effector T cells in co-culture and Transwell experiments were tested by flow cytometry. Serum of ALT was analyzed by automatic biochemistry analyzer and the injury of liver in ischemia reperfusion(IR) was detected by H&E staining. Results: Compared with the noM group, the expression of Foxp3 was reduced in the M0 group and the M2 group(both P < 0.01), while no significant difference was observed in the M1 group. What’s more,the suppressive ability of Tregs from the M0 and M2 group were less than those from the M0 and M2 group both in vitro and in vivo (both P < 0.01). Mechanism study proved that the regulatory effect of macrophage to Tregs was depended on cell-cell contact(P < 0.05). Conclusion: Our study suggests that peritoneal macrophages play an important role in regulating the induction of Tregs in vitro,which present key therapeutic potential in maintaining immune balance.

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王坤鹏,古 鉴,倪绪皓,赵 洁,汪 瀚,吕 凌.小鼠腹腔巨噬细胞对调节性T细胞体外诱导的影响[J].南京医科大学学报(自然科学版),2016,(9):1063-1067

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  • 收稿日期:2016-05-23
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  • 在线发布日期: 2016-09-18
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