CRYAB在喉癌中的表达变化及临床意义
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国家自然科学基金(81301951);江苏省六大人才高峰项目 (2015-WSN-017);南京市科委科技发展项目(201402055)


Differentiated expression and clinical significance of CRYAB in LSCC
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    摘要:

    目的:检测晶状体蛋白-αB(crystallin-alpha B,CRYAB)在人喉癌中的表达情况,同时研究CRYAB表达与喉癌重要临床指标之间的关系。方法:在10例新鲜喉癌及癌旁组织上进行一步法定量逆转录PCR(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)检测,在组织芯片上(80例喉癌标本及20例癌旁对照标本)进行免疫组化检测,使用Cox回归法及Kaplan-Meier生存分析法进行喉癌患者的预后评估。结果:qPCR及免疫组化结果均显示喉癌组织中的CRYAB表达明显高于癌旁对照组织中CRYAB的表达(P 均 < 0.05)。CRYAB的表达与喉癌患者的肿瘤分化程度(P = 0.022)-TNM分期(P = 0.013)-淋巴结转移(P = 0.026)及生存情况(P = 0.001)有重要关联。生存分析显示CRYAB表达(P = 0.036)与喉癌患者预后关系密切,CRYAB高表达提示患者预后不良。结论:CRYAB在喉癌组织中异常高表达,且与喉癌患者的数种恶性表型相关,可作为喉癌患者预后的独立危险因素。

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    Objective: To examine the expression of crystallin-alpha B (CRYAB) in human laryngeal squamous cell carcinoma(LSCC) and investigate the relationship between its expression and clinical characteristics of LSCC. Methods: One-step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) test in 10 fresh LSCCs as well as non-cancerous tissue samples and immunohistochemistry analysis by tissue microarrays (80 LSCC samples and 20 tumor-adjacent normal samples) were performed to characterize CRYAB expression in LSCC. Cox regression and Kaplan-Meier survival analyses were carried out to evaluate the prognosis of LSCC. Results: The results of qPCR test and immunohistochemistry analysis showed that the expression of CRYAB in LSCC was significantly higher than that in tumor-adjacent normal tissues(P = 0.0005 and P = 0.021, respectively). Moreover, the expression level of CRYAB protein in LSCC was significantly related to tumor differentiation(P = 0.022), TNM stage(P = 0.013),lymph node metastasis (P = 0.026) and overall survival (P = 0.001). COX multi-factor analysis showed that CRYAB expression (P = 0.036) was recognized as an independent prognosis factors for LSCC. Conclusion: The data suggest that CRYAB expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.

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许 里,徐新宇,林振中. CRYAB在喉癌中的表达变化及临床意义[J].南京医科大学学报(自然科学版),2016,(9):1095-1100

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  • 收稿日期:2016-02-23
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  • 在线发布日期: 2016-09-18
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