Objective:To investigate the clinical efficacy of litholysis by integrated traditional Chinese and Western medicine on rabbits with gallstone. Methods:A total of 40 New Zealand white rabbits were randomly divided into A,B,C and D group,with 10 in each group. They were given gallbladder fistula,placement of calculi,and indwelling fistula. Then they were respectively treated with Western medicine,Traditional Chinese,the combination of Traditional Chinese and Western Medicine,normal saline. The drug was injected by fistula,once every hour,72 hours continuously. After the end of the experiment,the residual stones were collected,cleaned and dried and weighed. The gallbladder,liver,kidney were taken pathological examination. Blood biochemical indexes of venous blood were examined. Results:Western medicine and the combination of traditional Chinese and Western medicine all have the obvious function of dissolving gallbladder stones. However,function damage of the liver and kidney and inflammatory response of the liver,gallbladder,kidney tissues of Western medicine group was larger,and that of combination of Western and traditional Chinese medicine was smaller,and that of traditional Chinese medicine was the smallest,but the function of dissolving gallbladder stones it was the weakest. Conclusion:The combination of traditional Chinese and Western medicine can not only keep the dissolution of gallbladder stones,but also reduce the adverse reactions.