Objective:To study the effecs of human trophoblast cell surface glycoprotein(Trop2) overexpression on migration of the human gastric mucosal epithelial cells and preliminary mechanism analyses. Methods:We explored mRNA and protein expression levels of Trop2 in GES-1 cell line by real-time RT-PCR and Western blot. OE-Trop2 and VE-Trop2 plasmids were constructed and then transfected into GES-1 cell line,and the efficiency of transfection was detected. Cell wound healing assay and Transwell migration assay were performed to detect the change of the migration in GES-1 after transfected OE-Trop2 plasmids. The change of surface markers(E-cadherin,fibronectin,and vimentin)of epithelial-mesenchymal transition(EMT)in GES-1 cell line were detected by Western blot after Trop2 overexpression. Results:Compared with gastric cancer cell lines BGC823 and MGC803,the mRNA and protein expression levels of Trop2 in GES-1 were lower. After OE/VE-Trop2 plasmid transfected in GES-1,the mRNA expression level of Trop2 in the OE-Trop2 group was 6.18 ± 0.52 times higher than that of the control group,whereas no differences were detected between the control group and the VE-Trop2 group. Meanwhile,the protein expression model of Trop2 was similar to that of the mRNA expression. After transfected plasmids 72 h,cell wound healing assay showed that the migration rate of GES-1 was 50% in the VE-Trop2 group and 95% in the OE-Trop2 group(P < 0.05). After transfected OE-Trop2 plasmids 24 h,transwell migration assay showed that the number of migration cell was 41 ± 6 in the VE-Trop2 group,whereas 87 ± 6 in the OE-Trop2 group(P < 0.05). Western-blot assay showed that the expression level of E-cadherin in GES-1 was down-regulated;meanwhile,fibronectin and vimentin were up-regulated after transfected OE-Trop2. Conclusion:Trop2 overexpression could promote migration of GES-1 cell line through inducing EMT phenomenon.