江苏人群12个非CODIS STR基因座的遗传多态性及在亲权鉴定中的应用
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Genetic polymorphism of 12 non-CODIS STR loci of Jiangsu population and its application in paternity testing
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    目的:利用3130遗传基因测序仪的电泳图谱分析江苏人群D11S4463-D2S1776-D14S1434-D4S2408-D10S1043-D20S482- D12SATA63-D19S433等12个非CODIS STR基因座多态性分布,计算该12个基因座的基因频率(P)-个体鉴别能力(DP)-无偏倚期望杂合度(H)-多态性信息总量(PIC)-二联体非父排除率(PEduo)和三联体非父排除率(PEtrio),并评估其法医学应用价值-方法:利用 PCR扩增,产物通过3130测序仪毛细管电泳-读出数据后用分析软件进行分析-结果:12个非CODIS STR 基因座的频率分布在本组人群中均符合Hardy-Weinberg平衡-杂合度分布为 0.707 6~0.804 6,个体鉴别能力为0.846 8~0.948 6,二联体非父排除率为0.278 6~0.482 6,三联体非父排除率为0.446 7~0.663 2,多态性信息总量为0.643 8~0.807 4-结论:通过大量的样本实验和等位基因频率的统计,得出等位基因多态性数据,用于比对验证,具有很好的辅助作用,在一些CPI值不足10 000及突变案例中具有重要的补充作用-

    Abstract:

    Objective:To analyze polymorphism distribution of 12 non-CODIS STR(including D11S4463,D2S1776,D14S1434,D4S2408,D10S1043,D20S482,D12SATA63,D19S433,etc.)of Jiangsu population by 3130 genetic gene sequencing electrophoretograms,and to calculate the gene frequency(P),discrimination power(DP),heterozygosity(H),polymorphic information content(PIC),probability of exclusion of due-testing(PEduo)and probability of exclusion of trios-testing (PEtrio). And then,to evaluate its forensic application value. Methods:The products of PCR amplification mere detected by capillary electrophoresis usiny 3130 sequenator. Data were analyzed by analysis software. Results:The frequency distributions of the 12 non-STR CODIS loci were consistent with the Hardy-Weinberg equilibrium. H ranged from 0.707 6 to 0.804 6,DP was 0.846 8 to 0.948 6,PEduo was 0.278 6 to 0.482 6,PEtrio was 0.446 7 to 0.663 2,and PIC was 0.643 8 to 0.807 4. Conclusion:Through the statistics of a large amount of experimental samples and allele frequencies,the allelic polymorphism data were obtained for comparison and verification. It has a good auxiliary function and play an important complementary role in cases of CPI less than 10 000 and mutation.

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居晓斌,潘 猛,刘燕婷,崔 鹤,周惠英,顾 民.江苏人群12个非CODIS STR基因座的遗传多态性及在亲权鉴定中的应用[J].南京医科大学学报(自然科学版),2016,(11):1348-1350

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  • 收稿日期:2016-09-03
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  • 在线发布日期: 2016-11-28
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