IL-8受体介导c-Jun和Ets-1异常调控MMP-9促进胃癌细胞侵袭
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广东省自然科学基金面上项目(S201310011923);惠州市科技计划项目(2013Y009)


IL-8 receptor mediated c-Jun and Ets-1 abnormal promoted gastric cancer cells metastasis through MMP-9
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    摘要:

    目的:探讨白介素-8(IL-8)受体促进胃癌侵袭所介导的分子机制。方法:胃癌细胞培养后分别转染空白质粒pcDNA3.1(+)-针对IL-8受体的反义表达质粒pcDNA3.1(+)/as-IL-8G和针对IL-8受体的正义表达质粒pcDNA3.1(+)/s-IL-8G,Western blot检测蛋白表达,细胞侵袭力用相对侵袭细胞数量表示。结果:低表达IL-8受体的胃癌细胞侵袭能力显著减弱,且细胞内c-Jun-Ets-1-MMP-9的蛋白水平显著下降;而高表达IL-8受体的胃癌细胞侵袭能力则显著增强,且细胞内c-Jun-Ets-1-MMP-9的蛋白水平显著上升。用硫代磷酸化修饰的反义寡聚脱氧核苷酸阻断该细胞内c-Jun或Ets-1的表达后,MMP-9的蛋白表达水平显著降低,并伴随细胞侵袭能力明显下降。结论:IL-8受体通过介导c-Jun和Ets-1异常调控MMP-9促进了胃癌细胞的侵袭过程。

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    Objective:To explore the molecular mechanism in which IL-8 receptor promoted gastric cancer metastasis. Methods:Gastric cancer cells were cultured and transfected with IL-8 receptor antisense expression plasmid pcDNA3.1(+)/as-IL-8G and IL-8 receptor sense expression plasmid pcDNA3.1(+)/s-IL-8G. Protein expression was detected by Western blotting. Cell invasion was performed in Transwell and the invasion capacity was expressed by the relative invasive cell number. Results:Gastric cancer cells with lower expression of IL-8 receptor had significantly reduced invasion ability,and the expression of c-Jun,Ets-1 and MMP-9 decreased correspondingly,while higher expression of IL-8 receptor markedly enhanced the invasion capacity of gastric cancer cells,and the expression of c-Jun,Ets-1 and MMP-9 increased significantly. MMP-9 protein expression level decreased significantly after blocking the c-Jun or Ets-1 expression by phosphorylated modification antisense oligonucleotide technology,and the cell invasion ability decreased obviously at the same time. Conclusion:IL-8 receptor promoted gastric cancer cells metastasis through abnormal c-Jun and Ets-1-mediated MMP-9.

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陈惠新,欧志英,陈丽莎,徐永成,曾书君,罗 程,余志金. IL-8受体介导c-Jun和Ets-1异常调控MMP-9促进胃癌细胞侵袭[J].南京医科大学学报(自然科学版),2017,(2):184-188

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  • 收稿日期:2016-02-13
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  • 在线发布日期: 2017-02-27
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