Objective:Based on the traditional transformation methods of polyethylene glycol/lithium acetate,the membrane permeability of Candida albicans cell was increased by modifying chemical treatment method,and further to change the efficiency of plasmid transformation. Methods:The recombinant plasmid was linearied using restriction enzyme,and then the transformatiom efficiency was detected through adding different concentrations of dimethyl suifoxide(DMSO)(1%,5%,and 10%)or taking various length of heat shock(0.5 h,2 h,3 h and 4 h)based on the traditional transformation methods. The optimum concentration of DMSO and the best length of heat shock were determined according to the transformation efficiency experiment. Finally,we cultured the Candida albicans on the plates of SC select medium using these two methods to compare the plasmid transformation efficiency between these two groups. Results:The efficiency of plasmid transformation using the common method is 0.6×105 transformants/1 μg plasmid DNA/108 cells,while the efficiency of that using the improved method is 1.5×105 transformants/1 μg plasmid DNA/108 cells. The differences between those two groups were statistically significant. Conclusion:The improved method increases the Candida albicans plasmid transformation efficiency.