二甲双胍对人血清诱导的胰腺癌细胞增殖及MMPs表达的影响
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国家高技术发展计划(863计划)(2015AA020314)


Effects of metformin on proliferation and expression of MMPs of cancer cells treated by human diabetic serum
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    摘要:

    目的:探讨二甲双胍对糖尿病患者血清诱导的胰腺癌细胞株Patu8988增殖的影响及相关基因表达的变化。方法:以糖尿病患者血清诱导细胞株Patu8988为糖尿病组,以健康人血清为对照组,用不同浓度的二甲双胍(2.5、5.0、10.0、20.0、40.0 mmol/L)干预两组细胞,通过CCK-8细胞增殖实验检测不同血清诱导后细胞的增殖能力,采用RT-PCR检测二甲双胍干预后基质金属蛋白酶(MMP)-2、MMP-8和MMP-11 mRNA表达的差异。结果:与健康对照组相比,糖尿病患者血清诱导后促进Patu8988细胞增殖(0.74±0.03 vs.0.62±0.02,P<0.01);二甲双胍抑制糖尿病患者血清诱导的细胞增殖(P<0.01);RT-PCR结果显示二甲双胍减弱MMP-2、MMP-8、MMP-11的mRNA表达。结论:二甲双胍抑制糖尿病患者血清诱导的细胞增殖,这种抑制效应可能与其对MMP-2、MMP-8、MMP-11mRNA表达的下调作用有关。

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    Objective:To observe effects of metformin on proliferation and related gene expression of human pancreatic cancer cell line Patu8988 treated by diabetic serum.Methods:Patu8988 cells were cultured with diabetic serum,or healthy human serum as control.The two groups were treated with different concentrations of metformin (2.5,5.0,10.0,20.0,and 40.0 mmol/L.The proliferation was measured by cell counting kit-8 (CCK-8) assay,and the expressions of matrix metalloproteinase-2 (MMP-2),matrix metalloproteinase-8 (MMP-8) and matrix metalloproteinase-11 (MMP-11) were examined by real-time PCR.Results:Compared with the control group,the proliferation of Patu8988 was promoted after treatment with diabetic serum (0.74±0.03 vs.0.62±0.02,P<0.01):Metformin inhibited the proliferation of Patu8988 treated with diabetic serum (P<0.01),and the expressions of MMP-2,MMP-8 and MMP-11 were downregulated.Conclusion:Metformin inhibits the proliferation of cancer cells induced by the serum from patients with diabetes,which may be related to the down regulation of MMP-2,MMP-8, and MMP-11.

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王琛琛,金 晖,施毕旻,孙子林.二甲双胍对人血清诱导的胰腺癌细胞增殖及MMPs表达的影响[J].南京医科大学学报(自然科学版),2017,(4):419-422

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  • 收稿日期:2016-06-12
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  • 在线发布日期: 2017-05-10
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