Objective：To analyze the genic characteristic of enterovirus 71(EV71) strains isolated from Jiangsu Province in 2014—2015. Methods：The EV71 RNA positive samples were inoculated into RD cells to separate the EV71 isolates，which were selected for sequencing and analysis of VP1 gene. The software DNA Star and MEGA 6.0 were used for comparison between EV71 and the various types of reference strains. Results：A total of 35 strains of EV71 were isolated and the positive rate was 29.17%. Sequence analysis of the 35 isolates showed that the homology of nucleotides and amino acids of VP1 gene were 93.3%～100.0% and 96.6%～100.0%，respectively. There were 95.6%～97.9% nucleotide acid identity and 97.9%～100% amino acid identity among these 35 strains and representative strain of C4a genotype isolated from Fuyang City. Phylogenetic analysis showed that the 35 EV71 strains gathered together with the representative strains of C4a genotype，and belonged to 2 viral transmission chains. Conclusion：The 35 EV71 strains from Jiangsu Province in 2014—2015 belong to C4a genotype. The major epidemic strain of EV71 in Jiangsu Province might have a same ancestor as domestic epidemic strains of EV71，and then evolve independently.