SLE患者CD4+CD25-T细胞来源的体外诱导型Treg细胞IL-10表达特征的初步研究
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国家自然科学基金(81401287)


Preliminary study about the expression of IL-10 on induced Treg cells from CD4+CD25- T cells of SLE patients in vitro
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    摘要:

    目的:探讨白细胞介素(interleukin,IL-10)在系统性红斑狼疮(systemic lupus ergthematosu,SLE)患者体外诱导型调节性T细胞 (induced regulatory T cell,iTreg)中表达的变化。方法:分离健康对照和SLE患者外周血单个核细胞,并进一步分离出CD4+CD25-T细胞,将其在体外以TGF-β诱导转化;用流式细胞术分析诱导转化后T细胞中CD25+和CD25-、Foxp3+和Foxp3-细胞亚群胞内IL-10(introcellular IL-10,iIL-10)和细胞膜表面IL-10(membrane IL-10,mIL-10)的表达。结果:①SLE患者和正常人经诱导转化的CD4+亚群细胞内CD25+T细胞表达iIL-10均明显增高,且患者显著高于正常人;②同样,患者和正常人诱导后CD4+亚群细胞内CD25+T细胞表达mIL-10均明显增高,但患者与正常人表达水平相似;③SLE患者和正常人经诱导转化形成的CD4+CD25+亚群细胞内Foxp3+T细胞表达iIL-10亦均明显增高,且患者显著高于正常人;④SLE患者和正常人经诱导转化形成的CD4+CD25+亚群细胞内Foxp3+T细胞表达mIL-10也明显增高,但患者和正常人类似;⑤SLE患者以上各项改变均与SLE疾病活动性指数无相关性。结论:正常人经诱导转化形成的CD4+CD25+Foxp3+Treg细胞与IL-10表达密切相关。SLE患者诱导转化后形成的iTreg细胞以及未转化的CD4+CD25-T细胞内表达iIL-10的水平均显著高于正常人;但是细胞膜上的mIL-10表达与正常人没有差异。

    Abstract:

    Objective: To investigate the level of IL-10 expressing on induced Treg cells(iTreg) such as CD4+CD25+T cells of SLE patients. Methods: Peripheral blood mononuclear cells(PBMCs) were isolated from healthy donors and SLE patients, respectively, and CD4+CD25-T cells were further isolated. Then the cells were induced and transformed by TGF-β in vitro; expression of intracellular and membrane IL-10 on CD25+ and CD25-,Foxp3+ and Foxp3- subsets of induced and transformed T cells were analyzed by flow cytometry. Results: Expression level of the intracellular IL-10(iIL-10) in CD25+ T cell subsets was obviously increased in both of the induced and transformed CD4+T cells from the normal controls and SLE patients, but it was significantly higher in the SLE patients. In the induced and transformed CD4+T cells both from the normal controls and SLE patients, expession level of the membrane IL-10 in CD25+ T cell subsets was obviously increased, while it had no difference between the SLE patients and the control group. In the normal subjects and SLE patients, the expession level of intracellular IL-10 in the induced and transformed CD4+CD25+ Foxp3+T cells subsets was obviously increased, and was significantly higher in the SLE patients than that of the control group. The expession level of membrane IL-10 was increased in the induced and transformed CD4+CD25+ Foxp3+T cells subsets, while no difference was observed between normal subjects and SLE patients. All the changes mentioned before have no correlation with SLEDAI. Conclusion: The IL-10 expression level was closely correlated with the induced and transformed CD4+CD25+ Foxp3+ Treg cells from the normal subjects. Abnormal expression of intracellular IL-10 was observed on the induced and transformed Treg cells from the CD4+CD25-T cell of SLE patients. The intracellular IL-10 exprssion level of CD4+CD25+Foxp3+ Treg cells from the SLE patients was significantly higher than that of the control group. And the intracellular IL-10 exprssion level of CD4+CD25+ Foxp3- Treg cells and CD4+CD25-T cell without induction was also significantly higher than that of the control group; But the expessions level of membrane IL-10 was not different between the SLE patients and the normal controls.

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赵 凌,杨晓帆,周小斌,季晓辉. SLE患者CD4+CD25-T细胞来源的体外诱导型Treg细胞IL-10表达特征的初步研究[J].南京医科大学学报(自然科学版),2017,(9):1114-1119

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  • 收稿日期:2017-03-13
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  • 在线发布日期: 2017-09-25
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