T1-T2双模式磁共振造影剂的制备及表征
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国家自然科学基金(81271629)


Preparation and characterization of T1-T2 dual-mode magnetic resonance contrast agent
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    摘要:

    目的:制备能同时实现T1-T2双模式磁共振成像的Fe3O4-BSAGd纳米粒子造影剂,并对其造影性能和生物相容性进行评价,以提高磁共振造影剂对病变部位诊断的敏感性及特异性,克服单一阳性造影剂和阴性造影剂的局限性,实现疾病的精准诊断。方法:采用高温热解法制备出油溶性粒径约6 nm的超顺磁性Fe3O4纳米粒子,随后利用超声乳化方式在其表面修饰顺磁性牛血清白蛋白-钆复合物(BSAGd),同时赋予Fe3O4纳米粒子顺磁性、水溶性和良好的生物相容性。并对其形貌、水合粒径(hydrated diameter,HD)、饱和磁化强度、弛豫效率(relaxation efficiency,r)、体外T1和T2加权成像效果、细胞毒性进行了表征。结果:Fe3O4-BSAGd纳米颗粒径为10 nm,水合粒径约32 nm,分散均匀稳定。Fe3O4-BSAGd的饱和磁化强度为30.2 emu/g。纵向(r1)及横向(r2)弛豫率分别为6.30 mM-1s-1和287.08 mM-1s-1。体外双模式成像结果显示其具有显著的T1-T2成像效果,并呈现浓度依赖性。细胞毒性测试结果显示,Fe3O4-BSAGd的生物相容性较好。结论:所制备的Fe3O4-BSAGd纳米粒子的体外T1和T2造影效果优良,生物相容性极好,为下一步的体内成像奠定了基础。

    Abstract:

    Objective:In order to improve the sensitivity and specificity of magnetic resonance contrast agent (MRCA) in the diagnosis of lesion site, and to overcome the limitations of single positive(T1) contrast agent and negative(T2) contrast agent as well as achieve the accurate diagnosis of diseases, a Fe3O4-BSAGd nanoparticle contrast agent was prepared for simultaneous T1-T2 dual-mode magnetic resonance imaging. The imaging performance and biocompatibility were evaluated. Methods:The superparamagnetic Fe3O4 nanoparticles with an oil-soluble particle size about 6 nm were prepared by the high temperature pyrolysis method, and the paramagnetic bovine serum albumin-gadolinium complex(BSAGd) was modified on the surface by phacoemulsification, while giving paramagnetic Fe3O4 nanoparticles, water solubility and good biocompatibility. Finally, the morphology, hydrated diametar(HD), saturation magnetization, relaxation efficiency(r), in vitro T1 and T2 weighted imaging and cytotoxicity were characterized. Results:The Fe3O4-BSAGd nanoparticles had a diameter of 10 nm and HD of about 32 nm, and the dispersion was uniform and stable. Fe3O4-BSAGd had a saturation magnetization of 30.2 emu/g. Longitudinal (r1) and transverse (r2) relaxation rates were 6.30 mM-1s-1 and 287.08 mM-1s-1, respectively. In vitro dual-mode imaging results showed a significant T1-T2 imaging effect and a concentration-dependent effect. The results of cytotoxicity test showed that the Fe3O4-BSAGd had a better biocompatibility. Conclusion:The effect of in vitro T1 and T2 weighted imaging of prepared Fe3O4-BSAGd nanoparticles was excellent with a good biocompatibility, which lays the foundation for the next step in vivo imaging.

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苏 伟,方向明. T1-T2双模式磁共振造影剂的制备及表征[J].南京医科大学学报(自然科学版),2017,(9):1168-1172

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  • 收稿日期:2017-04-19
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  • 在线发布日期: 2017-09-25
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