芹菜素逆转肺癌A549/DDP细胞耐药及机制
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

温州市科技局项目(Y20160403,Y20100257);浙江省中医药管理局项目(2009YB024)


The effect and mechanism of apigenin reverse drugs resistance on A549/DDP cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究芹菜素对肺癌A549/DDP细胞的作用,探讨芹菜素逆转肺癌耐药的作用及机制。方法:体外培养肿瘤细胞,以不同浓度芹菜素作用为实验组,用MTT法检测A549/DDP细胞增殖和分析药物敏感性,用Rhodamine-123潴留实验检测A549/DDP细胞内药物外排情况,用Western blot法检测肿瘤细胞内P-糖蛋白(P-glycoprotein,P-gp)和肺耐药蛋白(lung resistance-related protein,LRP)表达情况,用RT-PCR法检测肿瘤细胞内多药耐药基因(multidrug resistance gene l,MDR1)mRNA和LRP mRNA的转录情况。结果:与DDP组相比,20、40、80 μmol/L的芹菜素明显抑制了A549/DDP细胞生长增殖(P<0.05)。DDP对A549/DDP细胞的IC50为(14.33±0.41) μg/mL,在芹菜素作用下其IC50降为(5.76±0.36) μg/mL,逆转倍数为2.48,两者相比有统计学差异(P<0.05)。芹菜素作用下,A549/DDP细胞内的Rhodamine-123蓄积增加,细胞内P-gp表达减弱,MDR1 mRNA转录水平下降,对LRP表达及LRP mRNA转录无明显改变。结论:芹菜素对A549/DDP细胞生长有抑制作用;芹菜素还具有逆转A549/DDP细胞肿瘤耐药的功能,其机制与降低MDR1 mRNA转录和下调P-gp介导的药物外转功能有关。

    Abstract:

    Objective:To study the effect of apigenin on A549/DDP cells,and to explore the effect and mechanism of apigenin reverse drugs resistance on A549/DDP cells. Methods:The cells were cultured with different concentrations apigenin. A549/DDP cells proliferation and drug sensitivity was detected by MTT asssy. The drug extracellular transport was detected by Rhodamine-123 retention experiment, the P-gp and the LRP expression in the tumor cells was detected by Western blot, and the transcription of MDR1 mRNA and LRP mRNA in the tumor cells was detected by RT-PCR. Results: The apigenin can inhibit the A549/DDP cells proliferation. There were significant differences between the apigenin groups(20,40,80 μmol/L) and the DDP group(P<0.05). The DDP’s IC50 with the apigenin was (5.76±0.36) μg/mL in A549/DDP cells. The DDP’s IC50 without the apigenin was (14.33±0.41)μg/mL in A549/DDP cells, and the reversal index was 2.48. The apigenin can make high Rhodamine-123 accumulate in A549/DDP cells, down-regulate the P-gp expression in A549/DDP cells, and decrease the transcription of MDR1 mRNA in A549/DDP cells. Conclusion: The apigenin may inhibit the A549/DDP cells proliferation and to reverse drug resistance in A549/DDP cells, and the mechanism was ralated to decrease the MDR1 mRNA transcription and down-regulate the expression of P-gp that mediated drug extracellular transport.

    参考文献
    相似文献
    引证文献
引用本文

赵亚新,刘洁凡,钱海鑫.芹菜素逆转肺癌A549/DDP细胞耐药及机制[J].南京医科大学学报(自然科学版),2017,(11):1385-1388,1394

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-06-07
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-12-06
  • 出版日期:
通知关闭
郑重声明