兔下颌前牙即刻种植后种植体周围炎动物模型的构建
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国家自然科学基金(81472928);江苏省卫生计生委面上科研课题(H201641);江苏省青年医学人才项目(QNRC-2016850);江苏省“六大人才高峰”资助项目(2014-WSW-035);江苏省高校大学生创新计划项目(201310312020Z);江苏高校优势学科建设工程资助项目(2014-37)


Establishment of an experimental animal model of peri-implantitis after immediate implantation of mandibular anterior teeth in rabbits
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    摘要:

    目的:构建兔下颌前牙即刻种植后种植体周围炎动物模型。方法:选取12只健康雄性新西兰大白兔,麻醉后拔除兔下颌左侧前牙,在拔牙窝中即刻植入锥柱状纯钛种植体。待种植体植入8周后,随机选择3只实验兔处死,采用Micro-CT观察种植体骨结合状况。剩余实验兔随机分为3组:空白组不加刺激,对照组种植体周围注射磷酸盐缓冲溶液,实验组注射细菌内毒素溶液。加刺激2周后处死实验动物,取含种植体的下颌骨标本,采用Micro-CT观测骨量;取种植体周围牙龈标本,采用HE染色,观察牙龈组织病理学改变。结果:即刻种植8周后,种植体骨结合良好,牙龈健康。加刺激2周后,空白组和对照组牙龈健康呈粉红色,质地坚韧,种植体骨结合良好,种植体顶部骨组织无明显吸收,牙龈组织中见散在炎症细胞;实验组牙龈红肿,质地软,种植体顶部牙槽骨明显吸收,骨密度和骨体积分数显著降低,牙龈组织中见大量炎症细胞弥散性浸润。结论:种植体周围局部注射细菌内毒素可快速构建兔种植体周围炎原位动物模型。

    Abstract:

    Objective: To establish an experimental animal model of peri-implantitis after immediate implantation of mandibular anterior teeth in rabbits. Methods: A total of 12 healthy male rabbits were anesthetized in the study. A tapered titanium implant was inserted immediately after removal of the left mandibular anterior tooth in each rabbit. Three rabbits were randomly selected and executed after 8 weeks of implantation. Micro-CT was used to evaluate the osseointegration. Then, the rest of animals were randomly divided into three groups. The blank group was not given stimulation, while the control group and the experimental group were injected phosphate buffer solution and E.coli-LPS solution around the implant respectively. After 2 weeks of stimulation, all rabbits were sacrificed to collect the mandible samples with implants and gingival tissues. Micro-CT was used to evaluate bone mass around implants, and hematoxylin and eosin staining was used to observe the histological changes of gingival tissues. Results: After 8 weeks of implantation, satisfactory osseointegration of implants were formed with healthy gums. After 2 weeks of stimulation, the blank and control groups showed pink tough gums with scattered inflammatory cells and good osseointegration of implants without bone resorptions. The experimental group showed red swollen gums with massive inflammatory cells and apparent bone resorptions on the top of implants with reduced bone mineral density and percent bone volume. Conclusion: An experimental animal model of peri-implantitis in rabbits could be established rapidly by local injections of E.coli-LPS solution around the implant.

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虞颖娟,明盼盼,邱 憬.兔下颌前牙即刻种植后种植体周围炎动物模型的构建[J].南京医科大学学报(自然科学版),2017,(11):1515-1518,1523

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  • 收稿日期:2017-02-15
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  • 在线发布日期: 2017-12-06
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