低浓度过氧化氢诱导人黑素细胞提早衰老
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国家自然科学基金(81171517,81602774);江苏省“333”工程项目(LGY2016009);南京医科大学科技发展基金重点项目(2014NJMUZD044)


Low concentration of hydrogen peroxide⁃induced premature senescence in human epidermal melanocytes
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    摘要:

    目的:本研究旨在探讨氧化应激在人表皮黑素细胞衰老中的作用。方法:以不同浓度的过氧化氢(0、200、400、600、800、1 000 μmol/L)处理人黑素细胞5 d后,检测分析其细胞活力和凋亡。通过评估衰老相关指标β-半乳糖苷酶染色、细胞增殖、细胞周期分析、E-钙黏素以及衰老基因表达等改变,探讨低浓度过氧化氢(0~400 μmol/L)对黑素细胞衰老进程的影响。结果:与对照组比较,当过氧化氢浓度为200 μmol/L和400 μmol/L时,细胞活力和凋亡未见显著性改变;高浓度的过氧化氢(600~1 000 μmol/L)可以明显诱导黑素细胞凋亡;400 μmol/L的过氧化氢则可使黑素细胞出现以细胞树突缩短、增殖减慢、β-半乳糖苷酶蓝染率增加、细胞周期阻滞于G2期、E-钙黏素表达降低及衰老相关蛋白p53和p21的表达增加等改变。结论:400 μmol/L浓度的过氧化氢刺激能诱导黑素细胞提早衰老。

    Abstract:

    Objective:The aim of our research is to investigate the role of oxidative stress in cellular aging of human epidermal melanocytes. Methods:Cultured human melanocytes were subjected to different doses of H2O2(0、200、400、600、800、1 000 μmol/L)for 5 d,after which cell viability and apoptosis were assessed.Next we investigated whether exposure to low levels of H2O2(0~400 μmol/L)induces premature senescence via evaluating senescence-associated β-galactosidase staining,cell proliferation assay,cell cycle analysis,E-cadherin and senescent gene expression,etc. Results:Cell viability and apoptosis in melanocytes cultured with H2O2 at 200~400 μmol/L showed no significant difference as compared with cells cultured without H2O2 and apoptosis of melanocytes progressively increased at high concentrations of H2O2 (600~1 000 μmol/L). Treatment with 400 uM H2O2 tended to exhibit dendrite retraction,increased SA-beta-gal activity,declined rate of proliferation,G2 cell cycle arrest,and changes in the expression levels of p53 and p21.We further show that pre-senescent melanocytes express markedly reduced levels of E-cadherin. Conclusion:Melanocytes undergo premature senescence under the stimulation of 400 μmol/L concentration of H2O2 .

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孙 利,石家绮,丁书红,熊喜喜,李 雪,鲁 严.低浓度过氧化氢诱导人黑素细胞提早衰老[J].南京医科大学学报(自然科学版),2018,(2):180-184

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  • 收稿日期:2017-11-23
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  • 在线发布日期: 2018-03-02
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