脂氧素A4对高氧损伤肺泡Ⅱ型细胞系MLE⁃12的保护作用
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金资助(81300521,81270821,81370746)


Protective effect of lipoxin A4 on hyperoxia injury of typeⅡalveolar epithelial cell line MLE⁃12
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨脂氧素A4(LipoxinA4, LXA4)对高氧损伤肺泡Ⅱ型细胞系MLE-12的保护作用及机制。方法:体外传代培养MLE-12细胞,随机分为:空气组、高氧组、高氧+LXA4组、高氧+转化生长因了(TGF)-β1中和抗体组、高氧+LXA4+TGF-β1中和抗体组。倒置相差显微镜下观察各组MLE-12形态学变化;实时荧光定量PCR(qRT-PCR)法检测Ⅰ型胶原(collagenⅠ)、肌腱蛋白C(tenascin-C)、TGF-βR1、TGF-βR2和Smad3的mRNA水平。Western blot检测TGF-β1/Smads信号(TGF-βR1、Smad2、Smad3、Smad4、p-Smad2 和p-Smad3)蛋白表达。结果:①细胞形态:高氧组MLE-12明显失去原有正常细胞形态,细胞变圆,核固缩;药物干预组正常细胞数增多,大部分细胞形态与正常细胞形态基本相似;②细胞外基质(extracellular matrix,ECM)mRNA表达:LXA4和TGF-β1中和抗体能抑制高氧介导的collagenⅠ和tenascin-C mRNA的表达量升高(P < 0.05),其中高氧+LXA4+TGF-β1中和抗体组作用最为显著(P < 0.05);③TGF-β1/Smads信号mRNA和蛋白含量:LXA4和TGF-β1中和抗体能抑制高氧介导的TGF-βR1、TGF-βR2和Smad3的mRNA水平以及下调TGF-β1/Smads信号相关蛋白的表达量(P < 0.05),其中高氧+LXA4+TGF-β1中和抗体组细胞的表达量下调最为显著(P < 0.05)。结论:LXA4对高氧损伤肺泡Ⅱ型细胞系MLE-12的保护可能与调控TGF-β1/Smads信号以及collagenⅠ和tenascin-C的表达有关。

    Abstract:

    Objective:To investigate protectory effects and possible mechanisms of lipoxin A4(LXA4)on the type Ⅱ alveolar epithelial cell line MLE-12 with hyperoxia injury. Methods:MLE-12 cells were cultured and divided into five groups randomly:I:air group,Ⅱ:hyperoxia group,Ⅲ:hyperoxia+ LXA4 group,Ⅳ:hyperoxia+ TGF-β1 neutralizing antibodies,Ⅴ:hyperoxia+ TGF-β1 neutralizing antibodies+ LXA4. The growth state and morphological changes of the cells were observed by the inverted phase microscope;the mRNA expression of collagen Ⅰ,tenascin-C,TGF-β1 receptor(TGF-βR1),TGF-βR2 and Smad3 were measured by real-time quantitative PCR;the protein levels of TGF-β1/Smads signaling pathway(TGFβR1,Smad2,Smad3,Smad4,p-Smad2 and p-Smad3)were detected by Western blot. Results:①Morphological change:the cells under hyperoxia lost their normal shapes and showed necrosis,while the cells cultured with LXA4 and/lor TGF-β1 neutralizing antibodies were normal appropriately. ②The mRNA expression of extracellular matrix(ECM):LXA4 and TGF-β1 neutralizing antibodies attenuated the mRNA expression of collagenⅠ,tenascin-C markedly(P < 0.05),which expressed in the group hyperoxia+TGF-β1 neutralizing antibodies + LXA4 decreased most obviously. ③The mRNA and protein levels of TGF-β1/Smads signaling pathway:Compared with that of the hyperoxia group,the mRNA expression of TGF-βR1,TGF-βR2 and Smad3 decreased(P < 0.05),while the protein level of TGF-β1/Smads signaling pathway had the same change in intervention group,and it in the the group hyperoxia+ TGF-β1 neutralizing antibodies +LXA4 decreased most obviously(P < 0.05). Conclusion:LXA4 can achieve protection of hy-peroxia-induced injury to MLE-12 which may be related to collagenⅠ, tenascin-C and TGF-β1 /Smads signaling pathway.

    参考文献
    相似文献
    引证文献
引用本文

李淑君,李冰洁,罗妍妍,卢红艳,吴升华,陈筱青.脂氧素A4对高氧损伤肺泡Ⅱ型细胞系MLE⁃12的保护作用[J].南京医科大学学报(自然科学版),2018,(2):185-190

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-02-13
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2018-03-02
  • 出版日期:
通知关闭
郑重声明