Objective：Calcium phosphate cement（CPC）scaffold is widely used in bone tissue engineering due to its good biocompatibility and osteoconduction properties. However，it needs further improvements to widen its application. In this study，CPC scaffold with gold nanoparticles（AuNPs）were used to promote the proliferation and osteogenic differentiation of dental pulp stem cells（DPSCs）. Methods：The morphology of AuNPs was detected by transmission electron microscope（TEM）. The cells were seeded on CPC，and cultured in media containing 5 μg/mL AuNPs. Those seeded on CPC and cultured in normal media was set as control. The cell adhesion was detected by fluorescent stain 4 h after seeding. The cell proliferation was detected by CCK-8，and the alkaline phosphatase（ALP）activity was measured. Alizarin red staining（ARS）was used to detect the mineral synthesis. Results：TEM image showed that AuNPs were spherical with 20 nm in average diameter. Fluorescent images showed no significant difference in cell adhesion between the two groups. Significant difference in cell proliferation was detected at 14 days（P < 0.05）. ALP activity of the AuNPs media group was significantly increased（P < 0.05），and the mineralization formation of DPSCs was also increased compared with that of cells in normal media（P < 0.05）. Conclusion：It was concluded that CPC scaffold with AuNPs can promote the proliferation and osteogenic differentiation of DPSCs.