Objective：To investigate the expression and significance of MAP4K4 in pancreatic cancer（PC）and study its effect on proliferation. Methods：GEPIA and Oncomine databases were retrieved to analyze the expression of MAP4K4 in pancreatic tumors and normal pancreatic tissues；GEPIA and OncoLnc databases were performed to assess the relationship between MAP4K4 level and the survival time for patients with PC. Immunohistochemistry was used to detect the expression of MAP4K4 in human pancreatic tumors and adjacent normal tissues，then correlation and survival analysis were conducted to investigate the relationship between MAP4K4 level and clinicopathological parameters. qRT-PCR and Western blot were used to detect the expression of MAP4K4 in human PC cell lines. Lentiviruses were used to infect CFPAC-1 and MIA PaCa-2 to construct the cell lines with MAP4K4 overexpression and interfering，then CCK-8 and colony formation assay were conducted to study the influence of MAP4K4 on proliferation. Results：GEPIA and Oncomine databases showed that the expression of MAP4K4 was significantly higher in human pancreatic tumors than that in normal pancreatic tissues（P < 0.01）. GEPIA and OncoLnc databases revealed that higher expression of MAP4K4 was significantly associated with poorer survival time of PC patients（P < 0.01）. Data derived from immunohistochemistry indicated that MAP4K4 level was higher in human pancreatic tumors than that in adjacent normal tissues and was correlated with tumor size and differentiation（P < 0.05）. Survival analysis showed that the survival time of patients with high level of MAP4K4 was significantly lower than that with low level（P < 0.05）. Cell lines with MAP4K4 overexpression and interfering were successfully constructed. MAP4K4 overexpression significantly promoted，whereas MAP4K4 knockdown inhibited PC cell proliferaton compared with controls respectively（P < 0.05）. Conclusion：The expression of MAP4K4 is up-regulated in PC and correlated with poor prognosis，and it plays an important role in promoting proliferation in vitro.