Objective：To investigate the effect of miR-448 on the proliferation of oral squamous cell carcinoma，and to screen and verify the relvelant genes. Methods：Using RT-qPCR to study the expression of miR-448 in human OSCC. Then miR-448 inhibitor was transfected into OSCC cell line Cal-27 cells，and the effects of miR-448 inhibitor on proliferation and migration of OSCC were detected by MTT assay and scratch test. In addition，we screened the target gene of miR-448 by three gene prediction software，and verify the regulation of miR-448 on target genes. Results：We found that miR-448 was significantly increased in 15 pairs of OSCC tissues by RT-qPCR analysis. Compared with the control group，the proliferation and migration of Cal-27 cells were significantly decreased after transfected with miR-448 inhibitor. The expression of SPARCL1 gene was increased detected by RT-qPCR and Western blot，and the presence of site-specific binding of miR-448 to the SPARCL1 gene in the 3′ UTR region was confirmed by luciferase reporter. Conclusions：MiR-448 is highly expressed in clinical samples of oral squamous cell carcinoma. miR-448 down-regulates the expression of SPARCL1 by binding to SPARCL1-3′ UTR，playing an oncogene role in oral squamous cell carcinoma and promoting tumor cell proliferation and migration.