钛表面碱热处理构建聚多巴胺膜层对细胞黏附的影响
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国家自然科学基金(81670967);江苏省卫生厅面上项目(H201504);江苏省“六大人才高峰”第八批高层次人才项目(WSW?021)


The effects of alkali⁃heated titanium surfaces with dopamine adhering hydroxyapatite coating on the adhesion of cells
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    目的:研究碱热处理后多孔钛表面聚多巴胺黏附羟基磷灰石的复合涂层制备及其对大鼠骨髓间充质干细胞增殖、成骨分化能力的影响。评价碱热处理后多孔钛表面聚多巴胺黏附羟基磷灰石复合涂层作为种植体表面改性方法的作用。方法:通过多巴胺的自聚合在碱热处理后的多孔钛表面构建聚多巴胺(PDA)膜层,利用聚多巴胺的黏附性能将纳米级羟基磷灰石颗粒黏附至多孔钛表面,形成多孔钛表面聚多巴胺黏附羟基磷灰石的复合涂层,通过扫描电子显微镜(SEM)分析表面结构特征。分离培养SD大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs),采用SEM观察细胞黏附情况,并利用Cell Counting Kit-8(CCK-8)、碱性磷酸酶(alkaline phosphates,ALP)等检测其增殖、分化能力。结果:该改性方法成功在多孔钛表面形成聚多巴复合涂层。体外细胞实验表明该复合涂层对BMSCs生长无抑制作用,且对其黏附和早期增殖有明显促进作用。结论:碱热处理后多孔钛表面形成的聚多巴胺生物活性复合涂层对BMSCs的黏附和早期增殖有明显的促进作用。

    Abstract:

    Objective:To investigate the effect of bioactive composite layers on porous titanium(Ti)surfaces with dopamine adhering hydroxyapatite coating on rat bone marrow mesenchymal stem cells. Methods: Coating the Ti with dopamine,the modified surface to increase the hydroxyapatite(HA)’s adhesion onto the titanium implant surface which has porous structure treating with the alkali-heat to form bioactive composite layers. Scanning electron microscopy(SEM)and energy dispersive X-ray spectroscopy(EDX)were employed to investigate the surface evolution of titanium after the chemical surface treatment and HA adhesion. Bone marrow deriued stem cells(BMSCs) were cultured and initial cell attachment,morphology,proliferation were evaluated. Results:The bioactive composite layers on porous titanium surfaces with dopamine adhering hydroxyapatite coating was successfully prepared. The bioactive composite layers were applied in vitro,the growth of BMSCs was not inhibited on it. Conclusion:The bioactive composite layers significantly promote the adhesion and early proliferation of BMSCs in vitro. This finding shows that the proposed method demonstrates a good prospective application in surface modification of titanium.

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尤 莹,卜寿山,庄 海,陈飞扬,张继生.钛表面碱热处理构建聚多巴胺膜层对细胞黏附的影响[J].南京医科大学学报(自然科学版),2018,(6):833-837

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  • 收稿日期:2017-12-27
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  • 在线发布日期: 2018-06-22
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