Objective：To investigate the effect of bioactive composite layers on porous titanium（Ti）surfaces with dopamine adhering hydroxyapatite coating on rat bone marrow mesenchymal stem cells. Methods： Coating the Ti with dopamine，the modified surface to increase the hydroxyapatite（HA）’s adhesion onto the titanium implant surface which has porous structure treating with the alkali-heat to form bioactive composite layers. Scanning electron microscopy（SEM）and energy dispersive X-ray spectroscopy（EDX）were employed to investigate the surface evolution of titanium after the chemical surface treatment and HA adhesion. Bone marrow deriued stem cells（BMSCs） were cultured and initial cell attachment，morphology，proliferation were evaluated. Results：The bioactive composite layers on porous titanium surfaces with dopamine adhering hydroxyapatite coating was successfully prepared. The bioactive composite layers were applied in vitro，the growth of BMSCs was not inhibited on it. Conclusion：The bioactive composite layers significantly promote the adhesion and early proliferation of BMSCs in vitro. This finding shows that the proposed method demonstrates a good prospective application in surface modification of titanium.