Objective：To evaluate the capacity of cartilage extracellular matrix-derived scaffolds prepared by a modified method for constructing tissue engineered cartilage. Methods：The cartilage pieces were fragmented using two different methods to produce extracellular matrix（ECM） powder and fine extracellular matrix（FECM） powder. ECM scaffold and FECM scaffold were fabricated with ECM powder and FECM powder through freeing and lyophilization technique. Both of ECM and FECM scaffolds were seeded with bone marrow mesenchymal stem cells（BMSCs）as the control group and the experimental group. The poly（lactic-co-glycolic acid）（PLGA） scaffold were seeded with BMSCs induced to differentiate into chondrocytes as the positive control group. After cultured in vitro for 12 h，the cell-scaffold constructs were implanted in the nude mice subcutaneously. The cell-scaffold constructs were harvested and detected on histology and immunohistochemistry at day 28. Results：Cell-scaffold constructs of the experimental group grew much better than that of the control group，and similar to the postive control group. More cartilage-like tissues were generated using FECM scaffold and stained strongly for sGAGs and type Ⅱ collagen. Conclusion：FECM scaffold can promote the differentiation of BMSCs into chondrocytes without exogenous transforming growth factor-β1（TGF-β1） and have potential of application in tissue engineering cartilage .