Objective：The present study focuses on the effect of salubrinal on energy intake，body weight，blood glucose，serum insulin and morphological changes of pancreatic islets in gestational diabetic mice induced by high-sucrose/high-fat diet. Methods：Female C57BL/6J mice were divided into three groups randomly：normal control group（C），gestational diabetic model group（G）and salubrinal intervention group（G+S）. Energy intake，body weight，food intake and blood glucose were measured at multiple time points before and during pregnancy. At the 10th day of pregnancy，the blood was collected for the measurement of serum insulin levels. Then the scores of pancreatic β-cell function（HOMA-B）and insulin resistance（HOMA-IR）were calculated. At the 17th day of pregnancy，the mice were sacrificed，and the pancreas was collected for morphological examination. Results：The energy intake and body weight of group G and group G+S were respectively higher than that of group C at multiple time points before and during pregnancy，whereas there was little difference in these two parameters between group G and group G+S. After 1-month feeding，the tendency of blood glucose level was showed as：group G+S > group G > group C，and this tendency was more significant at the 10th day of pregnancy. However，there was no significant difference in blood glucose among the 3 groups at the 17th day of pregnancy. Although there was little difference in serum insulin levels among the 3 groups at the 10th day of pregnancy，HOMA-B showed：group G+S < group G < group C，and HOMA-IR showed：group G+S > group G > group C. Morphological examination showed that the morphology of pancreatic islets and the ultrastructure of pancreatic β cells in group C were normal. However，the number of pancreatic islets increased in group G and group G+S. The hyperplasia and atrophy of pancreatic islets occurred simultaneously in these 2 groups. Meanwhile，the abnormal ultrastructure of pancreatic β cells was characterized by severe swollen mitochondria and highly expansion of rough endoplasmic reticulum. Conclusion：These findings suggest that salubrinal has no potential for preventing and controlling gestational diabetic mice induced by high-sucrose/high-fat diet.