Objective：To investigate the related factors of anemia in patients with diabetic nephropathy（DN）diagnosed by renal biopsy. Methods：In this retrospective study，178 patients with DN diagnosed by renal biopsy were included. The levels of hemoglobin（Hb）<130 g/L（in males） and Hb<120 g/L（in females） were identified as anemia；The levels of Hb≥130 g/L（in males）and Hb≥120 g/L（in females）were identified as non-anemia. The relationship between anemia and clinical manifestations，laboratory findings，pathological classification of DN，renal tubular injury scores was analyzed. Logistic regression analysis was used to evaluate related factors in DN patients of different genders. Results：In 178 cases with DN，duration of diabetes（DD），estimation of glomerular filtration rate（eGFR），urinary albumin/creatinine（UACR），glycosylated hemoglobin（HbA1c），albumin（Alb），triglyceride（TG），low density lipoprotein cholesterol（LDL-C），serum iron（SI），transferrin（TF），high sensitive C reactive protein（hs-CRP）were significantly different between the anemia and the non-anemia group. There were significant differences in DD，eGFR，UACR，HbA1c，Alb，TG，SI，TF，hs-CRP between the two groups of male patients；in female cases，DD，Alb，HbA1c，UACR，hs-CRP were significantly different between the two groups. Logistic regression analysis showed that eGFR，Alb and DD，hs-CRP were related to anemia in male patients；Alb and hs-CRP were correlated with anemia in female patients. The tubulointerstitial damage score in the anemia group were significantly higher than that in the non-anemia group，and significant difference in Hb level between pathological stage Ⅱa and other stages was also detected. Conclusion：Related factors of anemia in DN patients include duration of diabetes，renal function deterioration，iron metabolism，inflammation and nutritional deficiency. Tubulointerstitial damage might be an important mechanism of renal function deterioration associated anemia.