MicroRNA⁃145在上皮性卵巢癌及细胞株中表达及其临床意义
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江苏省自然科学基金(BK20171304)


Expression and clinical significance of microRNA⁃145 in epithelial ovarian cancer tissues and cell lines
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    摘要:

    目的:研究microRNA-145(miRNA-145)在上皮性卵巢癌患者组织及血清中的表达情况,并探讨其作为肿瘤标记物在上皮性卵巢癌诊断和预后分析中的价值。方法:采用实时荧光定量逆转录聚合酶链反应检测45例上皮性卵巢癌患者和45例正常人血清标本中miR-145的表达,以及45例上皮性卵巢癌患者组织及8例正常卵巢组织中miR-145的表达。结果:miR-145在上皮性卵巢癌患者血清中相对表达量显著低于正常对照(P<0.01);miR-145在上皮性卵巢癌组织中的相对表达量明显低于正常卵巢组织(P < 0.01);miR-145表达水平随上皮性卵巢癌临床分期的进展呈下降趋势(P<0.05),与年龄、组织分级、病理类型、淋巴结转移和CA125的升高无关(P>0.05);ROC曲线显示血清miR-145可作为上皮性卵巢癌的分子诊断靶点;Kaplan-Meier 生存曲线显示血清miR-145的表达水平与上皮性卵巢癌患者的无进展生存率及总生存率有关(P<0.05)。结论:miR-145在上皮性卵巢癌的发生发展中发挥抑癌基因作用,可能成为诊断和预后评价的生物标志物。

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    Objective:To evaluate the expression of microRNA-145(miR-145)in epithelial ovarian cancer tissue and serum,and investigate the diagnosis and prognosis value of miR-145 as a biomarker for epithelial ovarian cancer. Methods:The expression of miR-145 in serum from 45 cases of epithelial ovarian cancer and 45 cases of healthy control,as well as in ovarian tissues from 45 cases of epithelial ovarian cancer and 8 cases of healthy control,was detected by real-time PCR. Results:The expressions of miR-145 in the serum and ovarian tissues of patients with epithelial ovarian cancer were significantly lower than those in healthy control(P<0.01). The expression of miR-145 decreased with the progression of the clinical stages(P<0.05),but it is not correlated with age,histological classification,lymph node metastasis,histological grading and the increasing of CA125(P>0.05). ROC curves revealed that serum miR-145 might be a promising diagnostic biomarker for patients with epithelial ovarian cancer. Conclusion:MiR-145 act as a tumor suppressor in the development of epithelial ovarian cancer. MiR-145 may serve as a biomarker for diagnosis and predictor of epithelial ovarian cancer.

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刘 萍,邹 晨. MicroRNA⁃145在上皮性卵巢癌及细胞株中表达及其临床意义[J].南京医科大学学报(自然科学版),2018,(11):1528-1532

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  • 收稿日期:2018-02-27
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  • 在线发布日期: 2018-12-03
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