Right ventricular function evaluation by 7.0T cardiac MRI in a rat model of pulmonary artery hypertension
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摘要:
目的：基于7.0T心脏磁共振成像（cardiac magnetic resonance，CMR）观察肺动脉高压（pulmonary artery hypertension，PAH）大鼠肺动脉径线和心脏功能的变化。方法：选用36只雄性SD大鼠（随机分为6组，每组6只）采用低氧建立PAH大鼠模型。于未缺氧时（基线）及缺氧后第1、2、3、4、5周利用MR（Bruker BioSpec 7.0T）进行心脏成像，分别测量主肺动脉（main pulmonary artery，MPA）、右肺动脉（right pulmonary artery，RPA）、左肺动脉（left pulmonary artery，LPA）直径，并计算其与同层面升主动脉（ascending aorta，AA）直径之比（MPA/AA、RPA/AA、LPA/AA）；四腔心位右室舒张末期最大径线（right ventricular end-diastolic maximum diameter，dRVmax）与左室舒张末期最大径线（left ventricular end-diastolic maximum diameter，dLVmax）之比（dRVmax/dLVmax），右室舒张末容积（right ventricular end diastolic volume，RVEDV），右室收缩末容积（right ventricular end systolic volume，RVESV），进而计算右室射血分数（right ventricular ejection fraction，RVEF）；每只大鼠均行右心导管术获取右室收缩压（right ventricular systolic pressure，RVSP）。采用SPSS 24.0统计软件，应用独立样本t检验及ANOVA分析比较不同时间点上述各参数的差异，采用Pearson相关性分析评价各参数与RVSP的相关性。结果：造模后第1周大鼠RVSP即较基线升高［（29.92 ± 1.94）mmHg vs.（41.55 ± 3.14）mmHg，P＜0.01］，后每周逐步升高，MPA、dRVmax/dLVmax及RVESV、RVEDV逐渐增大（P＜0.01），RVEF在缺氧第2周显著降低（P＜0.01）。MPA/AA、dRVmax/dLVmax、RVEF与RVSP具有较好的相关性（r分别为0.573、0.700、-0.760，P均＜0.01）。结论：7.0T CMR可敏感地观察到PAH大鼠病程进展中肺动脉及右心形态功能的改变，为研究PAH的演变机制打下基础。
Abstract:
Objective：To observe the evolution of pulmonary artery diameters and cardiac functional changes in a rat model of pulmonary artery hypertension（PAH）using 7.0T cardiac magnetic resonance（CMR）. Methods：A rat model of PAH was established by hypoxia. A total of 36 rats were divided into 6 groups on average（baseline，hypoxia at 1st，2nd，3rd，4th，and 5th weeks；n=6）. CMR was performed every week by Bruker BioSpec 7.0T. The diameters of the main pulmonary artery（MPA），right pulmonary artery（RPA），left pulmonary artery（LPA），right ventricular end-diastolic maximum diameter（dRVmax），left ventricular end-diastolic maximum diameter（dLVmax），right ventricular end-diastolic volume（RVEDV）and right ventricular end-systolic volume（RVESV）were measured on MR images；ratio of dRVmax to dLVmax（dRVmax/dLVmax），ratio of the main pulmonary artery to the ascending aorta（MPA/AA，RPA/AA，and LPA/AA），right ventricular and left ventricular ejection fraction（RVEF）were calculated. Right ventricular systolic pressure（RVSP）was obtained by right heart catheterization after CMR imaging for each rat. Comparison of above-mentioned parameters at different time points was calculated by t test and ANOVA analysis（SPSS 24.0）. Pearson correlation analysis was used to evaluate the correlation between above-mentioned parameters and RVSP. Results：The RVSP in model rats at the first week increased significantly compared with the baseline［（29.92 ± 1.94）mmHg vs.（41.55 ± 3.14）mmHg，P＜0.01］，and then gradually increased from the first week to the fifth（P < 0.01）. MPA，dRVmax/dLVmax，RVESV，and RVEDV gradually increased（P＜0.01）. RVEF decreased significantly in the 2nd week of modeling（P < 0.01）. There were moderate correlations between the MPA/AA，dRVmax/dLVmax，RVEF and RVSP in model rats（r=0.573，r=0.700，r=-0.760，all P < 0.01）. Conclusion：7.0T CMR can sensitively observed changes in pulmonary artery diameter and right ventricular function during the progression of PAH rats，which lays a foundation for studying the evolutionary mechanism of PAH.
