m6A识别蛋白Ythdf1在精子发生中的作用研究
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国家自然科学基金(31622039);江苏省自然科学基金(BK20160045)


Effects of m6A reader Ythdf1 during spermatogenesis
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    摘要:

    目的:构建YTH N6甲基腺苷RNA结合蛋白1(YTH N6-methyladenosine RNA binding protein 1,Ythdf1)的基因敲除小鼠,研究其在小鼠精子发生中的作用。方法:利用CRISPR/Cas9敲除技术及原核注射技术构建Ythdf1基因敲除小鼠模型,通过免疫荧光、HE染色对敲除小鼠睾丸及附睾进行形态学分析,通过交配实验进行生殖力测试,研究Ythdf1基因对雄性小鼠精子发生的影响。结果:RT-PCR结果显示,Ythdf1在1~8周龄雄鼠睾丸中均有表达;成功构建Ythdf1基因敲除小鼠;成年敲除小鼠精子发生、附睾及生育力均无异常。结论:在正常饲养情况下,敲除Ythdf1基因对雄性小鼠精子发生无影响。

    Abstract:

    Objective:Construct YTH N6-methyladenosine RNA binding protein 1(Ythdf1) knockout mice and study its role during mouse spermatogenesis. Methods:Ythdf1 knockout mice were generated by microinjection of CRISPR/Cas9 into zygote successfully. The role of Ythdf1 in mouse spermatogenesis was detected by immunofluorescence,HE and fertility test. Results:The results of RT-PCR showed that Ythdf1 was expressed in the testis of 1 to 8-week-old male mice. Ythdf1 knockout mice were successfully generated. These knockout mice showed normal spermatogenesis,epididymis morphology and fertility. Conclusion:This study found that deletion of Ythdf1 gene had no effect on spermatogenesis under normal breeding conditions.

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刘媛媛,宋小玲,王仿竹,齐美杰,沈 彬. m6A识别蛋白Ythdf1在精子发生中的作用研究[J].南京医科大学学报(自然科学版),2019,(2):171-175

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  • 收稿日期:2018-09-07
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  • 在线发布日期: 2019-03-06
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