花姜酮对小鼠CD4+CD25+Treg细胞分化功能的影响及机制
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

南京市卫生计委杰出青年基金(JQX16028)


The effects of Zerumbone on the differentiation and functions of CD4+CD25+Treg cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:建立小鼠CD4+CD25+Treg细胞的体外诱导及培养方法,同时探讨花姜酮(Zerumbone)对Treg细胞的分化、分泌功能的影响及其机制。方法:从BABL/c小鼠的脾脏分离、纯化CD4+CD62L+T细胞,加入转化生长因子(transforming growth factor,TGF)- β(5 ng/mL)、 白细胞介素(interleukin,IL)-2(30 μg/mL)促进CD4+CD62L+T细胞向Treg细胞分化。Treg细胞被分为5组:正常组、模型对照组、Zerumbone(1 μmol/L)组、Zerumbone(10 μmol/L)组、Zerumbone(30 μmol/L)组。流式细胞术检测各组Treg细胞的比例,酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)方法检测IL-10含量,Foxp3、IL-10 mRNA的表达用实时荧光定量聚合酶链反应(RT-PCR)方法检测。结果:本实验方法使Treg细胞的诱导比例明显升高(P< 0.01)。与模型对照组相比,Zerumbone组的Treg细胞比例呈剂量依赖性升高(P均< 0.05)。IL-10蛋白的表达与模型对照组比较也呈剂量依赖性升高(P均< 0.05),Foxp3、IL-10 mRNA的表达同样升高(P < 0.05),其中Zerumbone(30 μmol/L)组升高最明显(P < 0.01)。结论:在体外实验中,Zerumbone可以促进BABL/c小鼠体内的CD4+CD62L+T细胞向Treg细胞分化,并促进IL-10蛋白的表达,其机制可能与诱导CD4+CD25+Treg特异性转录因子Foxp3的表达有关。

    Abstract:

    Objective:To explore an induced and culture method for CD4+CD25+Treg cells in vitro,study the effect of Zerumbone about the differentiation and secretion functions of Treg cells and explore the mechanisms included. Methods:CD4+CD62L+T cells were isolated from BALB/c mice spleen and purified with magnetic bead methods. CD4+CD62L+T cells were co-cultured with transforming growth factor(TGF)-beta(5 ng/mL),interleukin(IL)-2(30 μg/mL) for CD4+CD25+Treg polarization.The cultured CD4+CD25+Treg cells were divided into five groups:the normal group;the induced group,which were cultured with the above protocol;Zerumbone(1 μmol/L)group;Zerumbone(10 μmol/L)group; Zerumbone(30 μmol/L)group. Flow cytometry was used to detect the proportion of CD4+CD25+Treg cells. The ELISA method was detected the levels of IL-10. Reverse transcriptase polymerase chain reaction(RT-PCR)was detected the level of IL-10 mRNA and Foxp3 mRNA. Results:The proportion of CD4+CD25+Treg cells cultured with the protocol were significantly higher compared with the normal group(P < 0.05). The CD4+CD25+Treg cells proportion in Zerumbone(1 μmol/L),Zerumbone(10 μmol/L),Zerumbone(30 μmol/L)groups were significantly increased compared with group model,there is dose dependent(P < 0.05). The protein level of IL-10 was increased by Zerumbone and that was also dose-dependent. Zerumbone increased the expression of IL-10 mRNA and Foxp3 mRNA(P < 0.05). Conclusion:Zerumbone can increase the differentiation of splenic CD4+CD62L Zerumbone into CD4+CD25+Treg cells and induce the expressions of IL-10 protein in vitro. The results may be thought the activation of Foxp3 in CD4+CD25+Treg cells.

    参考文献
    相似文献
    引证文献
引用本文

陈菲菲,毛 山,史 莹,周丹阳,张金梅,谷 伟.花姜酮对小鼠CD4+CD25+Treg细胞分化功能的影响及机制[J].南京医科大学学报(自然科学版),2019,(2):205-209

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-09-17
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-03-06
  • 出版日期: