Objective：To investigate the differential expression profile and bioinformatics analysis of microRNA（miRNA） in hepatic stellate cells（HSCs） activated by platelet-derived growth factor BB（PDGF-BB） inhibited by psalmotoxin-1（PcTx-1），blocker of acid-sensing ion channel 1a（ASIC1a）. Methods：HSCs in logarithmic growth period were divided into the control group，the model group and the PcTx group. The control group was not treated，after the PcTx group was stimulated by PcTx-1 for 1 h，the model group and the PcTx group were cultured with PDGF-BB（10 ng/mL）medium for 24 h. RT-PCR and Western blot were used to detect the expression of α-smooth muscle actin（α-SMA），collagen-Ⅰ and ASIC1a，and MTT was used to detect the proliferation of cells. These results verified whether HSCs were activated and whether PcTx-1 reduced the expression of ASIC1a. High throughput sequencing of total RNA was carried out after quality control，and differential miRNAs were screened. The target genes of differential miRNAs were predicted by miRanda algorithm，then the functional significance of miRNA target genes and metabolic pathway involved in miRNA were analyzed. Results：After blocking ASIC1a by PcTx-1，the expression of ASIC1a decreased，and the expressions of α-SMA and collagen-Ⅰ in HSCs activation were obviously reduced. It suggested that PcTx-1 can block the activation of HSCs induced by PDGF-BB. In the miRNA expression profile of PDGF-stimulated HSCs，there were 38 differentially expressed miRNAs，of which 6 were up-regulated and 32 were down-regulated（P < 0.05）. After ASIC1a was blocked by PcTx-1，there were 17 differentially expressed miRNAs in the miRNA expression profiles between the PcTx group and the model group，including 1 up-regulation and 16 down-regulation（P < 0.05）. Conclusion：The differential expression profile of miRNA in HSCs activated by PDGF-BB inhibited by PcTx-1 in high-throughput screening，providing new targets and ideas for the study of pathogenesis of liver fibrosis.