Objective:To compare the effect of ice water cooled cycle microwave ablation and normal temperature water cooled cycle ablation on the morphology of ablation area. Methods Nine pieces of fresh isolated porcine livers,weighing 4.5-5.2 kg. The experiment was divided into ice water cooled cycle group and nomral temperature water cooled cycle group. Each liver was ablated with normal temperature water cycle and ice water cooled cycle at the same time,at different ablation power(40,60,80 W),with different duration(5,8,10 min). After ablation,the liver tissues were cut along the needle path. The longitudinal diameters(LD),transverse diameters(TD) and preshoot diameters(PD) of the ablation areas were measured,and the spherical ratios(SR) were calculated. Results:Descriptive approach was used for preliminary reporting. Compared with the normal temperature group,the LD of the ice water group was relatively shortened by 13%,10%,12%,15%,11%,13%,8%,8%,while the TD and PD were not obviously changed,so the SR was relatively increased by 22%,21%,7%,14%,1%,1%,11%,5%,4%. In addition,at the same power,the improvement of SR was more obvious in short duration time(5 min). While at the same duration time,the improvement of SR was more obvious at low power(40 W). Conclusion:This experiment preliminarily hints that microwave ablation with ice water cold cycle may increase the SR by shortening the LD of the ablation area,especially in the case of low power and short time. But further experiments are needed to obtain more accurate results.