Objective：This study aims to observe the changes of intestinal function in 11β- hydroxysteroid dehydrogenase（11β-HSD1）knockout mice. Methods：Six 11β-HSD1 knockout mice were paired with 6 control mice with C57/BL6 genetic background both fed standard chow ad libitum for a period of 6-8 weeks. The changes in mice characteristics and intestinal morphometry were observed. The intestinal function and inflammatory alterations were determined by PCR and immunohistochemistry. Results：11β-HSD1 gene abortion significantly decreased the intestinal inflammation. Intestinal mucosal permeability was altered，intestinal villus length was shortened，crypt depth became deeper，goblet cell number increased，and mucosal barrier function was enhanced. Conclusion：This study successfully established a 11β-HSD1 knockout mice model，which showed inflammation amelioration in intestinal tract after 11β-HSD1 gene knockout，providing a research foundation for further specific knockouting 11β-HSD1 in intestinal tissue.